Flow Cytometry data from the R/V TINRO, NOAA Bell M. Shimada and CCGS Sir John Franklin during the 2022 International Year of the Salmon Pan-Pacific Winter High Seas Expedition
Citation
Eisner L B, Lomas M W (2023). Flow Cytometry data from the R/V TINRO, NOAA Bell M. Shimada and CCGS Sir John Franklin during the 2022 International Year of the Salmon Pan-Pacific Winter High Seas Expedition. Version 1.29. Hakai Institute. Sampling event dataset https://doi.org/10.21966/j26w-by50 accessed via GBIF.org on 2024-07-16.Description
Phytoplankton community composition and size structure vary considerably between oligotrophic and eutrophic regions (areas of low or high macro and micronutrients (e.g., iron)) (Hill et al., 2005; Martin et al., 1989; Strom et al., 2006; 2016), between surface and subsurface depths (Hill et al., 2005), and with season (Moran et al., 2012) and climatic conditions (Batten et al., 2021). Phytoplankton represent the base of the food web providing energy for zooplankton, which in turn support the growth of juvenile and adult salmon populations. Certain phytoplankton, like many diatom species, are particularly important food items in the Gulf of Alaska (GOA, Odate 1996, Strom et al., 2007). We aim to broaden understanding of phytoplankton dynamics in the GOA/North Pacific Ocean by investigating spatial and temporal patterns in community structure and biomass and exploring environmental (physics and nutrients) drivers of taxonomic variability that may lead to variation in the quality of phytoplankton biomass available to primary consumers during winter. The spatial variations in phytoplankton biomass, and taxa and community size structure were characterized through measurements of (among others) flow cytometry data collected at all IYS stations. In zone 4 (US Ship NOAA Bell M. Shimada) flow cytometry samples were collected from 5, 25, and 50 m for assessment of microbial community cell sizes (Moran et al. 2012). Flow cytometry were also collected at 5 m depths in zone 5 (on Canadian ship CCGS Sir John Franklin) and in zones 2-3 (on Russian ship R/V TINRO), so we have samples over the entire area surveyed. The flow cytometry analysis deliverables include tabulated counts (cells/ml) and estimated carbon content (C/cell and C/population) for the following 4 phytoplankton pico- and nanoplankton: Synechococcus, Cryptophytes, picoeukaryotes, nanoeukaryotes (excluding Cryptophytes).Sampling Description
Study Extent
Sampling took place at 88 stations in the North Pacific Ocean, from 2022-02-05 to 2022-03-20. The data includes samples done at various depths by three vessels: R/V TINRO, NOAA Bell M. Shimada and the CCGS Sir John Franklin.Sampling
The NOAA Bell M. Shimada flow cytometry samples were collected from 5, 25, and 50 m for assessment of microbial community cell sizes (Moran et al. 2012). Flow cytometry samples were also collected at 5 m depths by the CCGS Sir John Franklin (in duplicates) and the R/V TINRO.Method steps
- Filter one vial of the 10% paraformaldehyde (PFA) using a filter and syringes into a clean 5ml Eppendorf tube (conical shape) prior to using. You may need to thaw PFA in fridge before filtering. Use 2 vials of unfiltered PFA – wait to filter the next vial until the filtered PFA is used up. Store filtered PFA in the fridge. Label 2 mL cryovials tubes. Pipette two 750 ul aliquots of sample (using 1ml pipette) into appropriately labeled 2 mL cryovials. In the hood, wearing safety glasses, lab coat and nitrile gloves, add 75 microliters 10% PFA (using 100ul ‘dead’ pipette) to each 2 mL cryovial. Dispose of used tip in a plastic bag taped to the hood. Place the cryovials in the fridge in a temporary cryobox or tray for ~ 2 hours, and then transfer the cryovials into a labeled cryobox in the -80C freezer for storage (they can be frozen at -40C if a -80C freezer is not available. Additional information on sampling protocol used can be found in Casey et al. (2013, DOI: http://dx.doi.org/10.1016/j.dsr2.2013.02.002) and Moran et al. (2012, DOI: 10.1016/j.dsr2.2012.02.011). The flow cytometry analysis deliverables include tabulated counts (cells/ml) and estimated carbon content (C/cell and C/population) for the following 4 phytoplankton pico- and nanoplankton: Synechococcus, Cryptophytes, picoeukaryotes, nanoeukaryotes (excluding Cryptophytes).
Taxonomic Coverages
Geographic Coverages
North Pacific Ocean
Bibliographic Citations
Contacts
Lisa B. Eisneroriginator
position: Research Oceanographer
National Oceanic and Atmospheric Administration
Seattle
Washington
US
email: lisa.eisner@noaa.gov
homepage: http://www.noaa.gov/
Mike W. Lomas
originator
position: Marine Biogeochemist
Bigelow Laboratory for Ocean Sciences
Boothbay
US
email: mlomas@bigelow.org
homepage: https://www.bigelow.org/
userId: https://orcid.org/0000-0003-1209-3753
Lisa B. Eisner
metadata author
position: Research Oceanographer
National Oceanic and Atmospheric Administration
Seattle
Washington
US
email: lisa.eisner@noaa.gov
homepage: http://www.noaa.gov/
Tim van der Stap
distributor
position: Research Technician
Hakai Institute
Hakai Institute PO Box 25039
Campbell River
V9W 0B7
British Columbia
CA
email: tim.vanderstap@hakai.org
userId: https://orcid.org/0000-0002-0053-0795
Brett Johnson
distributor
position: Research Technician
Hakai Institute
Hakai Institute PO Box 25039
Campbell River
V9W 0B7
British Columbia
CA
email: brett.johnson@hakai.org
userId: https://orcid.org/0000-0001-9317-0364
Lisa B. Eisner
administrative point of contact
position: Research Oceanographer
National Oceanic and Atmospheric Administration
Seattle
Washington
US
email: lisa.eisner@noaa.gov
homepage: http://www.noaa.gov/
Mike W. Lomas
administrative point of contact
position: Marine Biogeochemist
Bigelow Laboratory for Ocean Sciences
Boothbay
US
email: mlomas@bigelow.org
homepage: https://www.bigelow.org/
userId: https://orcid.org/0000-0003-1209-3753