Antarctic Microbial Biodiversity(AMBIO) samples
Citation
Wilmotte A, Pessi I, Heughebaert A (2022). Antarctic Microbial Biodiversity(AMBIO) samples. University of Liège. Sampling event dataset https://doi.org/10.15468/h4vkp2 accessed via GBIF.org on 2024-08-31.Description
This dataset describe sampling events for the BelSPO project Antarctic Microbial Biodiversity (AMBIO) project which aims to determine the microbial diversity in Antarctic aquatic ecosystems, using an integrated and standardised analysis. The samples, collected between 1997 and 2007, were further analysed with metabarcoding techniques during the CCAMBIO project. eDNA derived occurrences to be published in sibling datasets.Purpose
This dataset only covers the geographical, temporal and environmental data of the samples. Occurrences datasets, derived from the analysis of these Antarctic samples, are expected to be published in sibling datasets and will refer to the samples published here.Sampling Description
Study Extent
The lake samples came from 5 regions of Antarctica - Eastern Antarctica: Larsemann Hills, Broknes Peninsula (L59b) and Fiilla Island (Rauer2) - Eastern Antarctica: Vestfold Hills (Highway, Waterfall) and Bunger Hills (M5) - Transantarctic Mountains: Forlidas Valley in Dufek Massif (Forlidas pond) and Shackleton Range (Lundström lake) - Antarctic Peninsula: Horseshoe Island (Col1 lake) - Lützow-Holm Bay: West Ongul, East Ongul, Langhovde, Skarvsness,Sampling
In Mago Lake, surface sediments were sampled at a depth of 5.8m using a UWITEC gravity corer (UWITEC, Mondsee, Austria). In the remaining lakes, samples were taken in the littoral zone at ca. 20 cm depth and ca. 40 cm from the lake edge, by manually collecting microbial mats attached to rocks and gravel. All samples were kept frozen (−20◦C) until analysis (Pessi et al. 2018). In the Larsemann Hills, for lake LH59b when no ice cover was present (lakes <2 m deep), samples were taken between 0.5 and 1 m in the littoral zone. All samples were frozen until analysis and stored in the dark (Sabbe et al.2004). For Rauer2, live samples of microbial mat (including cyanobacteria, diatoms and other micro algae) were collected from the littoral zone of the lake and stored under light in sterile culture tubes containing growth media. Samples of microbial mats were also frozen for use in community composition studies (Hodgson et al. 2001). For Forlidas pond, biological material was collected manually from lake littoral. Samples for microscopic investigation were preserved in Lugol’s iodine solution or ethanol and those for molecular analysis were frozen. All sampling equipment was scrubbed with Virkon multi-purpose disinfectant before use, and subsamples were collected in sterile WhirlPak bags or acid-washed bottles (Hodgson et al. 2010). For Lutzow-Holm Bay, sediment cores were extracted using a UWITEC gravity corer for surface sediments (Verleyen et al. 2017)Quality Control
The samples were taken as sterily as possible.Method steps
- See publications: Sabbe et al. 2004 10.1111/j.1365-2427.2004.01186.x. Hodgson et al. 2001 https://doi.org/10.1017/S0954102001000372 Hodgson et al. 2010 doi:10.1016/j.polar.2010.04.003 Verleyen et al. 2017 http://dx.doi.org/10.1016/j.quascirev.2017.06.003 Pessi et al. 2018 doi: 10.1093/femsec/fiy042
Taxonomic Coverages
all sort of microorganisms
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Bacteriarank: domain
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Cyanobacteriarank: phylum
Geographic Coverages
Antarctic continent
Bibliographic Citations
Contacts
Annick Wilmotteoriginator
position: Senior Research Associate (Life Sciences)
University of Liege
BE
homepage: https://www.cip.uliege.be/cms/c_6288624/en/cyanobacterial-diversity-phylogeny-and-biogeography
userId: http://orcid.org/0000-0003-3546-3489
Igor Pessi
originator
position: PhD Postdoctoral Researcher
University of Helsinki
FI
userId: http://orcid.org/0000-0001-5926-7496
André Heughebaert
metadata author
position: IT manager
Belgian Biodiversity Platform
BE
email: a.heughebaert@biodiversity.be
userId: http://orcid.org/0000-0002-7839-5300
Annick Wilmotte
principal investigator
University of Liege
BE
email: awilmotte@ulg.ac.be
userId: http://orcid.org/0000-0003-3546-3489
Wim Vyverman
administrative point of contact
Gent University Protistologie en Aquatische Ecologie (PAE)
BE
email: Wim.Vyverman@UGent.be
userId: http://orcid.org/0000-0003-0850-2569
Anne Willems
administrative point of contact
Gent University Laboratorium voor Microbiologie (LM-Gent)
BE
email: anne.willems@ugent.be
userId: http://orcid.org/0000-0002-8421-2881
Annick Wilmotte
administrative point of contact
position: Senior Research Associate (Life Sciences)
University of Liege
Allée du 6 aout, 13
Liège
4000
BE
Telephone: +3243663387
email: awilmotte@uliege.be
homepage: https://www.cip.uliege.be/cms/c_6288624/en/cyanobacterial-diversity-phylogeny-and-biogeography
userId: http://orcid.org/0000-0003-3546-3489