Artificial stream sediment raw sequence reads

Sampling

This study used artificial stream mesocosms to assess the effects of a single addition of nano-TiO2 (P25 at a final concentration of 1 mg L-1) on the abundance, activity and community composition of sediment-associated bacterial communities. The addition of nano-TiO2 resulted in a rapid (within one day) decrease in bacterial abundance in artificial stream sediments, but bacterial abundance returned to control levels within 3 weeks. Pyrosequencing of partial 16S rRNA genes did not indicate any significant changes in the relative abundance of any bacterial taxa with nano-TiO2 treatment, indicating that nano-TiO2 was toxic to a broad range of bacterial taxa and that recovery of the bacterial communities was not driven by changes in community composition.

Method steps

1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1