Incubation of marine methane seep sediments with a bioorthogonal amino acid
Citation
MGnify (2019). Incubation of marine methane seep sediments with a bioorthogonal amino acid. Sampling event dataset https://doi.org/10.15468/1unq0h accessed via GBIF.org on 2024-12-11.Description
In marine sediments, anaerobic oxidation of methane (AOM) is primarily catalyzed by syntrophic consortia of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB). We applied bioorthogonal non-canonical amino acid tagging (BONCAT) using a high-throughput approach to detecting protein synthesis in individual cells, on sediments from deep-sea methane seeps. Sediment samples have been incubated in the presence or absence of methane and a bioorthogonal amino acid. V6 regions of bacterial and archaeal 16S rRNA genes were amplified using general primers, tagged, and analyzed for shifts in the microbial community during time of incubation.Sampling Description
Sampling
In marine sediments, anaerobic oxidation of methane (AOM) is primarily catalyzed by syntrophic consortia of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB). We applied bioorthogonal non-canonical amino acid tagging (BONCAT) using a high-throughput approach to detecting protein synthesis in individual cells, on sediments from deep-sea methane seeps. Sediment samples have been incubated in the presence or absence of methane and a bioorthogonal amino acid. V6 regions of bacterial and archaeal 16S rRNA genes were amplified using general primers, tagged, and analyzed for shifts in the microbial community during time of incubation.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
- Hatzenpichler R, Connon SA, Goudeau D, Malmstrom RR, Woyke T, Orphan VJ. 2016. Visualizing in situ translational activity for identifying and sorting slow-growing archaeal-bacterial consortia. Proc Natl Acad Sci U S A vol. 113 - DOI:10.1073/pnas.1603757113
Contacts
originatorCalifornia Institute of Technology
metadata author
California Institute of Technology
administrative point of contact
California Institute of Technology