New Larix-associated Suillus species (Boletales, Agaricomycetes) from North Asia

Study Extent

The dataset contains annotations of specimens used for comparative and phylogenetic study, as well as their images and links to DNA sequences in the GenBank

Sampling

Collections were made, documented and preserved using the standard methods (Bondartsev, Zinger, 1955, Tulloss, 1999, Halling, 1996)

Quality Control

All determinations could be checked online or by the request of specimens from the LE and MAG. DNA sequences are deposited in Genbank.

Method steps

1. Specimens were photographed in situ, examined for taste and smell, dried at temperatures below 50°C.
2. Macroscopic descriptions were based on the study of both fresh and dried materials as well as on photographs.
3. Microstructures were observed at ×400 and ×1000 in squash preparations in 5% KOH, Congo Red, and Melzer’s reagent. Up to 30 basidiospores, 10 hymenial cystidia, and 10 terminal elements of the pileipellis per specimen were measured to obtain descriptive statistics.
4. Measurements were made in ADFImageCapture calibrated by OMP object-micrometre (LOMO). Dimensions are given as (absolute min) average min–average max (absolute max) of the all examined specimens, Q = average min–average max quotient (length/width ratio).
5. PCR ITS products were obtained without DNA extraction using the standard protocol of Thermo Scientific Phire Tissue Direct PCR Master Mix kit.
6. For amplification of the ITS region the primers ITS1-F, ITS4-B (Gardes, Bruns, 1993) were used.
7. LSU (28S) was amplified with LR0R, LR5 (Vilgalys, Hester, 1990) pair of primers, TEF1 with EF1-983F and EF1-1567R (Rehner, Buckley, 2005).
8. Amplified products were sequenced using BigDyeH Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, California).
9. The sequences were assembled in CodonCode Aligner V.9.0.1 (CodonCode Corporation).

13 specimens, 5 species, 1 variety, 1 form

1. Suillus gastroflavus
   rank: species
2. Ixocomus flavus
   rank: species
3. Suillus nueschii var. caerulescens
   rank: variety
4. Suillus bresadolae f. flavogriseus
   rank: form

Specimens originated from Italia (Suillus bresadolae f. flavogriseus (Cazzoli & Consiglio) Klofac MCVE18244 Ex Herb Simonini N1844) and Russian Federation from Altay Mountains (Suillus nueschii var. caerulescens Singer LE4547, LE4554), Irkutsk Region (Suillus sp. LE18056, LE18089, LE18421, LE4766) and Magadan Region (Suillus sp. MAG5111, MAG2642, MAG1307, MAG1308, Suillus gastroflavus Zvyagina, Rebriev, Sazanova & E.F. Malysheva Holotype MAG3480 and Paratype MAG5122/LE312702)