Metagenome analysis of picoeukaryotes from surface seawater
Citation
MGnify (2018). Metagenome analysis of picoeukaryotes from surface seawater. Sampling event dataset https://doi.org/10.15468/a2js9s accessed via GBIF.org on 2024-08-24.Description
Surface seawater was collected at two sampling sites in Sendai Bay (C5: 141.083W, 37.976N; C12: 141.317W, 37.974N), Miyagi, Japan on October 7 and December 1 in 2013. Suspended particles were concentrated to 100 fold and divided into two subsamples: (1) mixied with DMSO(5%) and frozen to sore in liquid nitrogen, (2) kept non-frozen. Picophytoplankton cells were sorted by flow cytometry. The genomic DNA was extracted and amplified by whole genome amplification. Partial 18S rRNA gene of the eukaryotes was amplified and sequenced using GS junior.Sampling Description
Sampling
Surface seawater was collected at two sampling sites in Sendai Bay (C5: 141.083W, 37.976N; C12: 141.317W, 37.974N), Miyagi, Japan on October 7 and December 1 in 2013. Suspended particles were concentrated to 100 fold and divided into two subsamples: (1) mixied with DMSO(5%) and frozen to sore in liquid nitrogen, (2) kept non-frozen. Picophytoplankton cells were sorted by flow cytometry. The genomic DNA was extracted and amplified by whole genome amplification. Partial 18S rRNA gene of the eukaryotes was amplified and sequenced using GS junior.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
- Kataoka T, Yamaguchi H, Sato M, Watanabe T, Taniuchi Y, Kuwata A, Kawachi M. 2017. Seasonal and geographical distribution of near-surface small photosynthetic eukaryotes in the western North Pacific determined by pyrosequencing of 18S rDNA. null vol. 93 - DOI:10.1093/femsec/fiw229
- Kawachi M, Kataoka T, Sato M, Noël MH, Kuwata A, Demura M, Yamaguchi H. 2016. Application of cryopreservation to genetic analyses of a photosynthetic picoeukaryote community. null vol. 576 - DOI:10.1016/j.gene.2015.10.033
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