Winter Picoplankton Diversity and Distribution in the US Antarctic Marine Living Resources Study Area - Northern Antarctic Peninsula
Citation
Murray A (2019). Winter Picoplankton Diversity and Distribution in the US Antarctic Marine Living Resources Study Area - Northern Antarctic Peninsula. Version 1.2. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/0zsina accessed via GBIF.org on 2024-09-02.Description
Picoplankton (bacteria, archaea, and eukaryotic organisms < 3.0 micron) surveys conducted in collaboration with the U.S. Antarctic Marine Living Resources (AMLR) field research program aimed to study the pelagic ecosystem during month-long winter research cruises in the South Shetland Islands, Scotia Sea and the NW Weddell Sea regions in August, 2012, 2013, and 2014. This inter-annual study on the winter pelagic ecosystem follows a 25-year time series program studying austral summer waters, though is the first survey to also include the characterization of the picoplankton in this study area. The primary goal of the AMLR winter cruise was to establish the ecological importance of winter processes and plankton distributons particularly as related to sea ice and to capture the hydrography and winter distributions of krill for comparisons with summer conditions over this long times series study. The winter time study will help determine how the template for ecological success is set up for summertime production across all trophic levels. The picoplankton research will improve the understanding of winter distributions and provide sample collection opportunities for diversity and biogeochemical studies to create an integrated ecosystem picture that will be developed with the NOAA AMLR group. In particular, this effort will extend the geographic coverage of winter picoplankton in the South Shetland Islands region, both on and off the continental slope. This is important to test hypotheses concerning high latitude winter processes that were observed in coastal Antarctic Peninsula waters suggesting the relative importance of chemolithoautotrophy is a key winter-time metabolic process.Sampling Description
Study Extent
The study was conducted in the US AMLR Study Area which covers coastal and off-shore waters in the vicinity of the South Shetland Islands and Elephant Island including the Bransifeld Strait, northern Weddell Sea, Weddell Sea confluence with the Scotia Sea, and Antarctic circumpolar current in Southern Drake Passage. We participated in three oceanographic polar winter cruises between August to early September in 2012, 2013, and 2014.Sampling
Hydrographic surveys were conducted using CTD rossette outfitted with niskin bottle samplers to retrieve water samples at 15, 50, 75, 100, 200, and 750 m (or above the bottom if bottom depth was shallower than 750 m). Water was filtered immediately once on board the ship in a cold room at 0C. Samples for DNA extraction and subsequent sequencing were passed through in-line 2.7 micron filters to remove larger organisms, and the picoplankton fraction was collected on 0.2 micron Sterivex cartridge filters. A sucrose-Tris-EDTA buffer was added to the filters and they were stored frozen at -80C until extraction at our home institutions.Quality Control
Nucleic extraction blanks were carried out through amplification steps and sequence analysis quality control measures were followed on data analyzed. Oceanographic instruments were calibrated at SeaBird before the cruise and sample blanks were periodically run for chlorophyll and nutrient analyses.Method steps
- These details will be reported in the literature.
Additional info
marine, harvested by iOBISTaxonomic Coverages
Bacterial, archaeal, and eukaryotic diversity in seawater picoplankton (< 3.0 micron fraction) and the diversity of the sea ice microbial community were described based on small subunit ribosomal RNA amplicon sequencing.
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Bacteriarank: domain
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Archaearank: domain
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Eukaryarank: domain
Geographic Coverages
This is the U.S. Antarctic Marine Living Resources study area. Hydrographic surveys were conducted across three broad sub-areas in this region, around Elephant Island, in the Bransfield Strait, west of the South Shetland Islands.
Bibliographic Citations
Contacts
Alison Murrayoriginator
position: Research Professor
Desert Research Institute
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: +1-775-673-7361
email: alison.murray@dri.edu
homepage: http://www.dri.edu/alison-murray
Alison Murray
metadata author
position: Research Professor
Desert Research Institute
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: +1-775-673-7361
email: alison.murray@dri.edu
homepage: http://www.dri.edu/alison-murray
Alison Murray
principal investigator
position: Research Professor
Desert Research Institute
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: +1-775-673-7361
email: alison.murray@dri.edu
homepage: http://www.dri.edu/alison-murray
Carla Gimpel
author
position: Graduate Research Student
University of Hawai'i
1001 Pope Rd.
Honolulu
96822
HI
US
email: cgimpel@uh.edu
Christian Reiss
author
position: Research Oceanographer
NOAA Fisheries, Antarctic Ecosystem Research Division
8901 La Jolla Shores Dr
La Jolla
92037
CA
US
email: christian.reiss@noaa.gov
Alison Murray
administrative point of contact
position: Research Professor
Desert Research Institute
2215 Raggio Parkway
Reno
89512
NV
US
Telephone: +1-775-673-7361
email: alison.murray@dri.edu
homepage: http://www.dri.edu/alison-murray