Nesterenkonia sphaerica
- Dataset
- Nesterenkonia salmonea sp. nov. and Nesterenkonia sphaerica sp. nov., isolated from the Southern Atlantic Ocean
- Rank
- SPECIES
- Published in
- Zhang, Gaiyun, Wang, Lina, Xie, Fuquan, Pei, Shengxiang, Jiang, Li (2020): Nesterenkonia salmonea sp. nov. and Nesterenkonia sphaerica sp. nov., isolated from the Southern Atlantic Ocean. International Journal of Systematic and Evolutionary Microbiology 70 (2): 923-928, DOI: 10.1099/ijsem.0.003847
Classification
- kingdom
- Bacteria
- phylum
- Actinobacteria
- class
- Actinobacteridae
- order
- Actinomycetales
- family
- Micrococcaceae
- genus
- Nesterenkonia
- species
- Nesterenkonia sphaerica
description
Cells are Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci (diameter 0.7 – 0.8 µm) without any flagella. Colonies on MA are approximately 1 mm in diameter, opaque, smooth, circular with regular margins, convex and light-yellow in colour after 6 days at 30 ° C. Growth occurs in 0 – 9 % (w / v) NaCl (optimum, 1 – 4 %), at pH 7 – 12 (pH 9 – 10) and at 10 – 40 ° C (30 – 35 ° C). Positive for catalase and oxidase. Negative for nitrate reduction, methyl red test, production of H 2 S and melanin, Voges – Proskauer reaction, and hydrolysis of starch, cellulose and casein. Assays using the API ZYM system are positive for alkaline phosphatase, esterase (C 4), esterase lipase (C 8), leucine arylamidase and naphthol-AS-BI-phosphohydrolase; weakly positive for valine arylamidase and acid phosphatase; negative for N - acetyl- β - glucosaminidase, cystine arylamidase, α - chymotrypsin, α - glucosidase, lipase (C 14), trypsin, α - galactosidase, β - glucuronidase, β - galactosidase, β - glucosidase, α - mannosidase and β - fucosidase. Analysis using the API 20 NE system reveals that cells are positive for hydrolysis of aesculin and gelatin; negative for the remaining 18 substrates. The major menaquinones are MK- 7, MK- 8 and MK- 9. The peptidoglycan type is A 4 α, L-Lys – L-Glu. The polar lipids detected are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid, two unidentified phospholipids and three unidentified lipids. The major fatty acids are anteiso-C 15: 0 and anteiso-C 17: 0; in addition, significant amounts of iso-C 16: 0, iso-C 15: 0 and C 16: 0 are also present. The genomic DNA G + C content of the type strain is 64.3 mol %.
etymology
Nesterenkonia sphaerica (sphae'ri. ca. L. fem. adj. sphaerica spherical, referring to the cell shape of the type strain).
materials_examined
The type strain, GY 239 T = (KCTC 39640 T = MCCC 1 A 10688 T), was isolated from marine sediment collected from the Southern Atlantic Ocean.
Name
- Homonyms
- Nesterenkonia sphaerica