The InBIO Barcoding Initiative Database: Ypsolopha milfontensis, a new species from the Portuguese coast (Lepidoptera: Ypsolophidae)
Citation
Corley M, Ferreira S, Sousa P, Beja P (2022). The InBIO Barcoding Initiative Database: Ypsolopha milfontensis, a new species from the Portuguese coast (Lepidoptera: Ypsolophidae). Version 1.3. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/92cdan accessed via GBIF.org on 2024-08-15.Description
The dataset contains eight records of moth specimens (Lepidoptera, Ypsolophidae) collected in 2019 in continental Portugal (Corley & Ferreira, 2021). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted and larvae reared to adulthood, and by night using mercury-vapor lamps or UV light traps to attract the insects. Captured specimens were identified to species level and preserved in 96% ethanol or in dry storage. A tissue sample, a leg, was removed from each individual to be used for DNA extraction. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).Purpose
The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.Sampling Description
Study Extent
Specimens were collected in the districts of Bragança, Beja and Vila Real of continental Portugal. Sampling was conducted in 2019.Sampling
Sampling of specimens was done at four different localities of continental Portugal. Sampling was conducted in May and September of 2019. Captured specimens were preserved in 96% ethanol or in dry storage. A tissue sample, a leg, was removed from each individual and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.Quality Control
The specimens' morphological identification was contrasted with the BLAST results of the specimens' newly generated DNA barcodes in the BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).Method steps
- Sampling of specimens was done at four different localities of continental Portugal, in 2019. Adult specimens were detected by direct search and hand-netted during the day, and lured using mercury-vapor lamps or UV light traps by night. Ypsolopha milfontensis larvae were found feeding on Ephedra fragilis Desf (Gnetopsida, Ephedraceae), and reared to adulthood.
- Captured specimens were preserved in 96% ethanol or in dry storage.
- Specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available taxonomic literature.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).
Taxonomic Coverages
This dataset is composed of data relating to eight Lepidoptera specimens. All specimens were determined to species level. The dataset is composed of three species, Ypsolopha alpella (Denis & Schiffermüller, 1775), Y. milfontensis Corley & Ferreira, 2021 and Y. rhinolophi Corley, 2019. All species belong to the family Ypsolophidae Guenée, 1845.
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Animaliarank: kingdom
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Arthropodarank: phylum
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Insectarank: class
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Lepidopterarank: order
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Ypsolophidaerank: family
Geographic Coverages
Continental Portugal
Bibliographic Citations
- Corley, M.F.V. & Ferreira, S. 2021. Ypsolopha milfontensis Corley & Ferreira, a new species from the Portuguese coast (Lepidoptera: Ypsolophidae). SHILAP, 49(193): 55-64. -
Contacts
Martin Corleyoriginator
position: Researcher
Pucketty Farm Cottage
Faringdon
SN7 8JP
Oxfordshire
GB
Telephone: +351 252660411
email: martin.corley@btinternet.com
userId: http://orcid.org/0000-0003-4240-8007
Sónia Ferreira
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
Pedro Sousa
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656
Pedro Beja
originator
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Martin Corley
metadata author
position: Researcher
Pucketty Farm Cottage
Faringdon
SN7 8JP
Oxfordshire
GB
email: martin.corley@btinternet.com
userId: http://orcid.org/0000-0003-4240-8007
Sónia Ferreira
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
Pedro Sousa
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656
Pedro Beja
metadata author
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Pedro Beja
administrative point of contact
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Sónia Ferreira
administrative point of contact
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966