The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 02 - Limoniidae, Pediciidae and Tipulidae

Study Extent

Portugal, including the Autonomous Regions of the Azores and of Madeira.

Sampling

The studied material was collected in 83 different Portuguese localities. Sampling was conducted between 2003 and 2019, although 82% of the records were made in the years 2018 and 2019. Specimens were collected by direct search and individual netting of specimens, by sweeping the vegetation with a net or were directly collected at light traps, using both UV and mercury vapor lights. Captured specimens deposited in the IBI reference collection were preserved in 96% alcohol.

Quality Control

For all DNA barcoded specimens, their morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

Method steps

1. Specimens were collected in 83 different Portuguese localities. Field work was carried out between 2003 and 2019, with 82% of the records made in the years 2018 and 2019.
2. Specimens were collected during fieldwork by direct search and individual netting of specimens, by sweeping the vegetation or were directly collected at light traps (using both UV and mercury vapor lights) and preserved in 96% alcohol. The majority of captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
3. All specimens were morphologically identified using the available literature except seven that were identified using the BOLD Identification Engine . For some specimens it was necessary to prepare and then exam their terminalia.
4. All specimens were DNA barcoded. For these, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. All DNA extracts were deposited in the IBI collection.
5. All sequences in the dataset were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

This dataset is composed of data relating to 412 Diptera specimens. All specimens were determined to species level except for seven specimens. Overall, 83 species are represented in the dataset. Two species have been further identified to subspecies level, Nephrotoma suturalis wulpiana (Bergroth, 1888) and Nephrotoma appendiculata pertenua Oosterbroek, 1978. These species belong to 31 genera unevenly distributed among three Craneflies families, Limoniidae (21 genera), Tipulidae (4 genera) and Pediciidae (2 genera). The families Limoniidae and Tipulidae account for a similar number of collected specimens, 197 and 211, respectively. However, Limoniidae has a higher proportion of recorded species, 45, when compared with Tipulidae, with 36 species.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Diptera
   rank: order
5. Tipuloidea
   rank: superfamily
6. Limoniidae
   rank: family
7. Pediciidae
   rank: family
8. Tipulidae
   rank: family

Continental Portugal, Autonomous Regions of the Azores and of Madeira.