Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017)
Citation
Luo W, Ding H, Li H, Ji Z, Huang K, Zhao W, Yu Y, Zeng Y, Sweetlove M (2021). Microbial communities in colored snow from Fildes Peninsula (King George Island, Maritime Antarctica, 2017). SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/u6zfw7 accessed via GBIF.org on 2024-08-18.Description
Amplicon sequencing dataset (Illumina MiSeq) targeting Eukaryota (18S ssu rRNA) and Bacteria (16S ssu rRNA) in red or green colored snow samples (n=10) from Fildes Peninsula, Antarctica (2017).Sampling Description
Study Extent
Colored snow samples (500 mL) from Fildes Peninsula (Antarctica) taken on 2017-01-30.Sampling
Samples were taken aseptically, using a 30 × 30 cm colored snow squares for each sampling station.Method steps
- Samples were allowed to naturally melt (≤ 10 °C) and were subsequently filtered through a 0.2 μm nucleopore membrane filter (Whatman). The filters were frozen at − 80 °C in cetyltrimethyl ammonium bromide (CTAB) buffer until analysis. DNA extraction was performed as described by Luo et al. (2017). PCR of microbial eukaryotes targeted the V4 region of the 18S ribosomal RNA (rRNA) gene: forward primer 3NDf (5ʹ-GGCAAGTCTGGTGCCAG-3ʹ) and reverse primer V4_euk_R2 (5ʹ-ACGGTATCTRATCRTCTTCG-3ʹ). The V4–V5 hypervariable regions of the bacte- rial 16S rRNA gene were amplified using PCR with the universal primers 515F-Y (5ʹ-GTGYCAGCMGCCGCG GTAA-3ʹ) and 926R (5ʹ-CCGYCAATTYMTTTRAG TTT-3ʹ).
- PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany), and quality was examined with a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations and sequenced on an Illumina MiSeq2000 platform.
Taxonomic Coverages
Geographic Coverages
Antarctica:Fildes Peninsula
Bibliographic Citations
- Luo, W., Ding, H., Li, H., Ji, Z., Huang, K., Zhao, W., ... & Zeng, Y. (2020). Molecular diversity of the microbial community in coloured snow from the Fildes Peninsula (King George Island, Maritime Antarctica). Polar Biology, 43(9), 1391-1405. -
Contacts
Wei Luooriginator
Polar Research Institute of China
Shangai
CN
Haitao Ding
originator
Polar Research Institute of China
Shangai
CN
Huirong Li
originator
Polar Research Institute of China
Shangai
CN
Zhongqiang Ji
originator
Second Institute of Oceanography
Hangzhou
CN
Kaiyao Huang
originator
Chinese Academy of Sciences
Wuhan
CN
Wenyu Zhao
originator
CN
Yong Yu
originator
Polar Research Institute of China
Shangai
CN
Yinxin Zeng
originator
Polar Research Institute of China
Shangai
CN
Maxime Sweetlove
metadata author
Royal Belgian Institute of Natural Sciences
Brussels
BE
email: msweetlove@naturalsciences.be
Wei Luo
user
Polar Research Institute of China
Shangai
CN
Wei Luo
administrative point of contact
Polar Research Institute of China
Shangai
CN