Alkalinema pantanalense Vaz 2015
- Dataset
- Newly recorded genera and species, Pantanalinema rosaneae and Alkalinema pantanalense (Leptolyngbyaceae, Cyanobacteria) isolated in Korea
- Rank
- SPECIES
Classification
- kingdom
- Bacteria
- phylum
- Cyanobacteria
- class
- Cyanobacteriia
- order
- Leptolyngbyales
- family
- Leptolyngbyaceae
- genus
- Alkalinema
- species
- Alkalinema pantanalense
biology_ecology
Ecology: The species was first collected in the saline-alkaline lake of wetland in Pantanal, Brazil and named as Alkalinema pantanalense. Alkalinema pantanalense (CENA 528) was reported to survive in the range of pH 8.4 to 9.9 for growth experiment but brought about a change to 8.4 to 9.9 pH in the cultured medium (Vaz et al., 2015). In this study, it turned out to be an epilithic species growing on gravels in the freshwater, implying that they can be distributed in an extensive water environment. Site of collection: Anseongcheon, Anseong-si, Gyeonggi Province (37 ° 00 ʹ 04.2 ʺ N / 127 ° 16 ʹ 13.5 ʺ E). Date of collection: September 20, 2020. Specimen Locality: FBCC-A 1468, FBCC-A 1469 in the Nakdonggang National Institute of Biological Resources (NNIBR). Gene sequences: The 16 S rRNA to 23 S rRNA gene sequences: GenBank accession Nos. MZ 567048, MZ 56 7049. 16 S rRNA gene and phylogenetic affiliation of Alkalinema pantanalense The region from 16 S rDNA to 23 S rDNA were sequenced for two strains (MZ 567048, MZ 567049) of Alkalinema pantanalense. DNA analysis showed that the two strains had completely identical 16 S rRNA gene sequences (DNA similarity 99.7 %). The phylogenetic relationships of the genus Alkalinema and other relatives were investigated by using a ML tree constructed from their partial 16 S rRNA gene sequences. The additional Bayesian tree turned out to be quite close to the ML tree (Fig. 4). The strains of A. pantanalense (FBCC-A 1468, FBCC-A 1469) clustered with the type species A. pantanalense (CENA 528) and another strains of A. pantanalense (CENA 529, 530, 531), and are clearly identified to be the same species in the ML tree. The strain of A. pantanalense FBCC-A 1468 showed a 100 % DNA similarity to FBCC-A 1469 and 0.00 % p - distance between them. On the other hand, the strains of A. pantanalense (FBCC-A 1468, FBCC-A 1469) showed a 99.5 to 99.9 % DNA similarity to type species A. pantanalense (CENA 528) and 0.00 % p - distance between them (Table 1).
description
The trichomes do not have sheaths but do have a diffluent mucilage. Colonies are grown on interwoven mats. Cells are longer than they are wide, 2.2 - 3.1 μm long by 2.8 - 3.2 μm wide, with a narrowed apical cell. As above, morphological features of this strain were identical to the original description of the type species (Vaz et al., 2015).
Name
- Homonyms
- Alkalinema pantanalense Vaz 2015