Stylochus mistus Ramos-Sánchez, Carrasco-Rodríguez, García-Madrigal, and Bastida-Zavala 2021
- Dataset
- Marine flatworms (Platyhelminthes: Polycladida) found in empty barnacle shells including a new species, from southern Mexican Pacific
- Rank
- SPECIES
- Published in
- Ramos-Sánchez, Mariela, Carrasco-Rodríguez, Dahani S., García-Madrigal, María Del Socorro, Bastida-Zavala, J. Rolando (2021): Marine flatworms (Platyhelminthes: Polycladida) found in empty barnacle shells including a new species, from southern Mexican Pacific. Zootaxa 4965 (2): 301-320, DOI: 10.11646/zootaxa.4965.2.5
Classification
- kingdom
- Animalia
- phylum
- Platyhelminthes
- class
- Rhabditophora
- order
- Polycladida
- family
- Stylochidae
- genus
- Stylochus
- species
- Stylochus mistus
description
(Figures 2 A – F, 3 A – G, 4 A – G, 5 A – C)
description
Description of external features Color. The background color is beige with a scant orange to light brown pigmentation; black transverse spots which, when fusing together, give the appearance of lines, mainly in the anterior marginal region of the body; nuchal tentacles orange; body margin orange or pink (Figs 2 A – C). When fixed the color is beige, with maculae, spots and small dots black or olive green (Fig. 2 D); and when stained with Mayer´s carmalum it has a purple to pink color (Figs 2 E – F). Form. Oblong shape or slightly pyriform (after to fixed) (Figs 2 A – F, 3 A, 5 A), measuring between 2 – 9 mm long (n = 26, µ = 4.77 mm, SD = 1.79) and 1 – 5.2 mm (n = 26, µ = 2.77 mm, SD = 1.07) wide. Tentacles. Circular to pyriform nuchal tentacles (Figs 3 C, 5 A), positioned between 0.8 – 1.2 mm from the anterior margin of the body. They measure 0.25 – 0.3 mm in length (n = 4, µ = 0.27 mm, SD = 0.02) and 0.2 mm in width (n = 4, µ = 0.2 mm, SD = 0.02) and with 0.5 mm intertentacular distance. Eyes. About 90 to 295 (n = 4, µ = 156, SD = 94) marginal eyes (Figs 3 B, 5 A) distributed in the anterior marginal region of the body; between 15 – 49 (n = 4, µ = 28, SD = 16) tentacular eyes (Figs 3 B – C, 5 A) mostly located within the nuchal tentacles and one or two pairs of these located in the marginal-posterior region of the tentacles; between 26 – 59 (n = 4, µ = 35, SD = 13) cerebral eyes arranged in two lines (Figs 3 B – C, 5 A), located anterior to the brain and oriented towards the anterior region of the body; few frontal eyes (Fig. 3 B, 5 A) distributed towards the anterior region of the body, arranged slightly in a fan-like manner. Brain. Oval, measuring 0.3 mm in length and 0.2 mm width, with scarcely visible globuli cell masses, positioned in the anterior region of the brain (Fig. 3 D). Digestive system. Folded pharynx (Figs 3 E, 5 A), 2 – 3.6 mm long (n = 4, µ = 2.9 mm, SD = 0.76) and 1.2 – 2.2 mm (n = 4, µ = 1.5 mm, SD = 0.42) wide, located at 2.2 mm from the anterior margin of the body; with 6 – 7 (n = 4, µ = 6.8, SD = 0.4) pharyngeal lobes. The mouth is located at the end of the first third of the pharynx (Fig. 3 E). Gonopores. Separated gonopores, positioned at the end of the last third of the body; the male gonopore is at a distance of 5.7 – 6 mm from the anterior margin of the body, at 1.4 mm from the pharynx and 0.05 mm from the female gonopore (Figs 3 F – G, 4 A, 5 A – C). Description of reproductive features Male reproductive system. Male gonopore almost terminal; the rest of male reproductive system oriented towards anteriorly from male gonopore; it presents spermiducal ducts which are oriented towards the anterior region of the body and extend to the second third of the pharynx, to descend later to the posterior region of the body. Prostatic vesicle prominent, oval and polyglandular (Figs 4 A-B, E, 5 B – C), located dorsal to the seminal vesicle. Seminal vesicle elongated and prominent (Figs 3 E – F, 4 A – B, 5 C), connected through the spermiducal ducts to the spermiducal vesicles (Figs 3 G, 5 C). Penis papilla short (Fig. 4 C), 1 mm long, unarmed; narrow male atrium (Figs 4 – F) with a glandular epithelium lining (Figs 4 D – F) in the peripheral region. Female reproductive system. Oviducts (Figs 4 C, 5 C) run from the anterior region of the body, around the pharynx, and connect directly with the proximal end of the vagina. Scarcely developed, narrow and short vagina, oriented towards the anterior region of the body (Figs 4 C – D, 5 C). Cement glands (Fig. 5 C) are scarce and are found surrounding the vagina; lacks Lang’s vesicle. Habitat. The polyclads were found inside the shells of Paraconcavus pacificus (Fig. 2 G), which were part of the fouling of a marine sensor (SeapHOx ®), at a depth of approximately 20 – 25 m. The sensor was installed on December 6, 2016, and extracted on March 7, 2017; the dominant fouling fauna was represented by barnacles, decapods and polychaetes.
distribution
Distribution. Tropical Eastern Pacific. Central coast of Oaxaca: Tangolunda Bay, Bahías de Huatulco.
etymology
Etymology. The specific epithet, mistus, derives from the Zapotec subdialect word mistu’, which means “ cat ”, in reference to the color and dorsal color pattern of the specimens (Figs 2 A – C), a pattern similar to the wild cat Leopardus wiedii Schinz, distributed along the coast of Oaxaca. Taxonomic remarks: Du Bois-Reymond Marcus & Marcus (1968) established two subgenera within Stylochus: S. (Stylochus) and S. (Imogine), based on the shape of the seminal vesicle. The first subgenus has a simple thinwalled seminal vesicle, while S. (Imogine) has on anchor-shaped (tripartite) seminal vesicle. Jennings & Newman (1996) raised subgenus Imogine to the genus level. Based on this separation, the new species was placed within the genus Stylochus. In this way, the genus Stylochus is represented by 34 species (Tyler et al. 2006 - 2020), of which four were described from the Eastern Pacific: Stylochus atentaculatus Hyman, 1953, from San Mateo Beach, California; S. californicus Hyman, 1953, from California; S. franciscanus Hyman, 1953, from San Francisco Bay, California and S. insolitus Hyman, 1953, from San Pedro Breakwater, California. Prior to this study, the genus Stylochus had not been recorded in the Tropical Eastern Pacific. Seven of the species described in this genus are associated with barnacles: Stylochus alexandrinus Steinböck, 1937, from Alexandria, Egypt (Galleni 1976); S. ellipticus (Girard, 1850), from south of Massachusetts (Hyman 1940); S. neapolitanus (Delle Chiaje, 1841 - 1844), from Sicilia (Barnes 2002); S. pilidium (Goette, 1881), from Sevastopol Bay, Black Sea (Rzhepishevskii 1979); S. pygmaeus Merory & Newman, 2005, from Victoria, Australia (Merory & Newman 2005); S. tauricus Jacubowa, 1909, from the Black Sea (Murina et al. 1995), and S. zanzibaricus Laidlaw, 1903, from Zanzibar Island, Africa (Skerman 1960). The comparison of Stylochus mistus sp. nov. was made taking into consideration the external and internal characters of the four species from California as well as the seven species associated with the barnacles. Stylochus mistus sp. nov. has frontal eyes and marginal eyes distributed in the first third of the body; it differs from S. neapolitanus, S. pygmaeus and S. zanzibaricus in that those lack frontal eyes (Laidlaw 1903; Merory & Newman 2005; Marquina et al. 2014; Noreña et al. 2015), and from S. atentaculatus, S. californicus, S. insolitus, and S. tauricus because the marginal eyes are distributed over the entire body margin (Hyman 1953 a; Murina et al. 1995). Stylochus mistus sp. nov. has separate gonopores and an unarmed penis papilla, and differs from S. franciscanus, S. alexandrinus, species which have a common gonopore (Hyman 1953 a; Galleni 1976); and from S. ellipticus (Hyman 1939 a; Hyman 1940) because this species has a penis papilla with stylet. The species of the genus Stylochus can be differentiated based on the color and pattern of coloration, presence / absence of frontal eyes, the distribution of marginal eyes, the morphology of the hermaphroditic reproductive system, the presence / absence of armament of the penis papilla and the shape of the vagina (Table I) (Hyman 1939 a; 1940; 1953 a). Other taxonomic characters for the specific definition, suggested by the present study, are: 1) the internal arrangement of the prostatic vesicle, which may be of the polyglandular or monoglandular type (reviewed in Bulnes et al. 2005); 2) presence of a glandular lining of the male atrium, described previously only in S. ellipticus (Hyman 1939 a, 1940), and also present in Stylochus mistus sp. nov.
materials_examined
Type locality: Tangolunda, Bahías de Huatulco, Oaxaca, México (15 ° 46 ’ 15.8 ” N, 96 ° 5 ’ 43.2 ” W). Type material. Twenty-eight specimens. Holotype: as sagittal sections: UMAR-PLAT 40, reproductive structures in seven slides; Tangolunda Bay, Oaxaca, southern Mexican Pacific; in the empty shell of Paraconcavus pacificus, 20 – 25 m in SEAPHOX ®, March 07, 2017, Coll. CCB, MAAS, HMMO, PGRP, and SVM). Paratypes: Five specimens as whole mounts: UMAR-PLAT 41 A-D, 5 specs. (Tangolunda Bay, same as holotype). One specimen in histology sections, UMAR-PLAT 41 E, 1 spec. in the frontal section of reproductive structures in two slides (Tangolunda Bay, same as holotype). Other specimens reviewed: Twenty-one specimens preserved in 70 % ethanol: UMAR-PLAT 41 F, 13 specs. fixed in 96 % ethanol (damaged specimens, Tangolunda Bay, same as holotype); UMAR-PLAT 41 G, 8 specs. fixed in formalin 10 % (damaged specimens; Tangolunda Bay, same as holotype).