Survey of xoxF diversity in different marine environments
Citation
MGnify (2019). Survey of xoxF diversity in different marine environments. Sampling event dataset https://doi.org/10.15468/chivb8 accessed via GBIF.org on 2024-09-01.Description
Methanol dehydrogenase is a key enzyme in methylotrophic bacteria, catalysing the oxidation of methanol to formaldehyde. In many methylotrophs, this is a well-characterised pyrroloquinoline quinone dependent enzyme consisting of two subunits, encoded by the genes mxaFI. A homolog of the large subunit MxaF is also present in most known methylotrophs, encoded by the gene xoxF. In several methylotrophs, this gene was found to be the only putative methanol dehydrogenase, but it is also present in several non-methylotrophs. This has led to a discussion over its potential role in methylotrophy and C1 cycling. This study aims to investigate the presence and diversity of the xoxF gene in the marine environment.Sampling Description
Sampling
Methanol dehydrogenase is a key enzyme in methylotrophic bacteria, catalysing the oxidation of methanol to formaldehyde. In many methylotrophs, this is a well-characterised pyrroloquinoline quinone dependent enzyme consisting of two subunits, encoded by the genes mxaFI. A homolog of the large subunit MxaF is also present in most known methylotrophs, encoded by the gene xoxF. In several methylotrophs, this gene was found to be the only putative methanol dehydrogenase, but it is also present in several non-methylotrophs. This has led to a discussion over its potential role in methylotrophy and C1 cycling. This study aims to investigate the presence and diversity of the xoxF gene in the marine environment.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
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originatorUniversity of East Anglia
metadata author
University of East Anglia
administrative point of contact
University of East Anglia