Collection of biological samples - Maternal and fetal, 2015-16 Zika epidemic (Colombia)
Citation
Mercado Reyes M M, Benavides Ruiz M M, Alvarez Díaz D A, Corchuelo Chavarro S Y (2021). Collection of biological samples - Maternal and fetal, 2015-16 Zika epidemic (Colombia). Version 1.2. Instituto Nacional de Salud. Occurrence dataset https://doi.org/10.15472/v1nfb5 accessed via GBIF.org on 2024-08-24.Description
The observation of congenital malformations associated with vertical transmission (mother-fetus) of Zika Virus (ZIKV) such as microcephaly, made ZIKV a global threat to health, especially for child development. In this scenario, the Instituto Nacional de Salud (INS) - Colombia, established the collection of biological samples of pregnant women with ZIKV infection collected in the context of intensified surveillance during the 2015-16 Zika epidemic in Colombia. The samples were collected in three cities in Colombia that presented the highest number of cases: Cali, Barranquilla, and Cúcuta. From this surveillance, 461 pregnant women were included. The database and its samples rest in the COMMAFEZ collection of the INS.
La observación de malformaciones congénitas asociadas a la transmisión vertical (madre-feto) del virus del Zika (ZIKV) como la microcefalia, convirtió al ZIKV en una amenaza mundial para la salud, en especial para el desarrollo infantil. En este escenario, el Instituto Nacional de Salud – Colombia, estableció la colección de muestras biológicas de mujeres embarazadas con infección por ZIKV recolectadas en el marco de la vigilancia intensificada durante la epidemia del Zika en Colombia en el periodo 2015-2016. Las muestras fueron colectadas en tres ciudades de Colombia que presentaron el mayor número de casos: Cali, Barranquilla y Cúcuta. A partir de ésta vigilancia se incluyeron 461 gestantes. La base de datos y sus muestras reposan en la colección COMMAFEZ del Instituto Nacional de Salud.
Purpose
To ensure the storage and availability of biological material obtained during the Zika epidemic in Colombia (2015-2016), for research associated with the understanding of this diseaseSampling Description
Study Extent
Valle del Cauca (Cali) Atlántico (Barranquilla) Norte de Santander (Cúcuta)Sampling
Biological material was collected from pregnant women with suspected ZIKV infection who attended for medical consultation at Health Services Provider Institutions in the cities of Cali, Barranquilla, and Cúcuta. 2833 samples were cryopreserved in saline solution at -80°C, including umbilical cord 36%, placenta 35%, cord blood 11%, serum 10%, urine 5%, ovular membranes 1%, amniotic fluid 1% and other tissues 1%. 1762 samples of placental, cord and fetal tissues were Formalin-Fixed and Paraffin-Embedded (FFPE) and 3063 slides with tissue sections were processed for histopathological analysis.Quality Control
The samples were taken by trained biomedical personnel throughout the process, complying with the biosafety standards for the handling of the samples and following the INS Sample Collection instructions (INT-R03.318-006), which was authorized in the framework of the epidemiological surveillance of the Zika virus developed by the INS and the sample management protocol in the epidemiological surveillance mission studies carried out by the Technical Directorate of Surveillance and Risk Analysis. Also, the guidelines of the Manual for obtaining and sending samples for analysis of events of interest in public health carried out by the INS in 2011, were followed. Controlled vocabulary adjustment in: - Record element: type; - Geographical element: county.Method steps
- 1) Identification of symptomatic pregnant women: Pregnant women with suspected infection by ZIKV were notified to the public health surveillance system of INS SIVIGILA. Once identified, they were invited to participate in the follow-up of their pregnancy and their children until they were two years old. 2) Once the informed consent was signed, samples were taken of the placenta, umbilical cord, urine, amniotic fluid, and umbilical cord blood during delivery, in order to subsequently demonstrate the presence of Zika virus in any of these. 3) Placental Processing Surveillance program for pregnant women with Zika - sent in neutral buffered formalin. 4) Shipment of placental paraffin blocks in the framework of ZIKA research (VEZ program). 5) Histopathological analysis. 6) Data management: The information associated with the tissues rests physically in the file of the Maternal and Perinatal Health (INS) group and a magnetic medium database. Data collected from the clinical history and epidemiological notification form of SIVIGILA.
Taxonomic Coverages
-
Zika Viruscommon name: ZIKV rank: species
Geographic Coverages
Bibliographic Citations
- 1. Revista-Semana. La generación del zika: así viven los niños que nacieron en medio de la epidemia. Semana [Newsletter] 2019 April 04, 2019; Available from: https://www.semana.com/vida-moderna/articulo/la-generacion-del-zika-asi-viven-los-ninos-que-nacieron-en-medio-de-la-epidemia/610454. 2. Cuevas, E.L., et al., Preliminary Report of Microcephaly Potentially Associated with Zika Virus Infection During Pregnancy - Colombia, January-November 2016. MMWR Morb Mortal Wkly Rep, 2016. 65(49): p. 1409-1413. 3. Fernandez, M.P., et al., Ocular Histopathologic Features of Congenital Zika Syndrome. JAMA Ophthalmol, 2017. 135(11): p. 1163-1169. 4. Mercado-Reyes, M., et al., Dengue, chikungunya and zika virus coinfection: results of the national surveillance during the zika epidemic in Colombia. Epidemiol Infect, 2019. 147: p. e77. 5. Parra-Saavedra, M., et al., Serial Head and Brain Imaging of 17 Fetuses With Confirmed Zika Virus Infection in Colombia, South America. Obstet Gynecol, 2017. 130(1): p. 207-212. 6. Instituto Nacional de Salud. Circular externa 0020 de 2016. Nuevos lineamientos para el fortalecimiento de la vigilancia por laboratorio del virus Zika en el territorio Colombiano.www.ins..gov.co/Normativ1dad/Circulares/CIRCULAR%200063%20DE%202015.pdf. -
Contacts
Marcela María Mercado Reyesoriginator
position: Subdirectora de Investigación Científica y Tecnológica
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: 122077001315
email: mmercado@ins.gov.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
Monica Marcela Benavides Ruiz
originator
position: Líder de Laboratorio
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: 122077001315
email: marceb.ruiiz@gmail.com
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
Diego Alejandro Alvarez Díaz
originator
position: Profesional Especializado
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: +57122077001315
email: dalvarezd@ins.gov.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
userId: https://scholar.google.com/citations?user=ApWpYpwAAAAJ&hl=en
Sheryll Yohana Corchuelo Chavarro
originator
position: Profesional Especializado
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: 122077001315
email: scorchuelo@ins.gov.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
userId: http://scholar.google.com/citations?user=https://scholar.google.com/citations?hl=en&user=3LmURIwAAAAJ
Diego Alejandro Alvarez Díaz
metadata author
position: Profesional Especializado
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: (1)2207700 ext 1315
email: daalvarezd@unal.edu.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
userId: http://scholar.google.com/citations?user=https://scholar.google.com/citations?hl=en&user=ApWpYpwAAAAJ
Marcela María Mercado Reyes
principal investigator
position: Subdirectora de Investigación Científica y Tecnológica
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: 5+72207700
email: mmercado@ins.gov.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
Instituto Nacional de Salud
owner
position: Titular
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: +5712207700
email: contactenos@ins.gov.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
Diego Alejandro Álvarez Díaz
author
position: Profesional Especializado
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: +57122077001315
email: dalvarezd@ins.gov.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
userId: http://scholar.google.com/citations?user=https://scholar.google.com/citations?hl=en&user=ApWpYpwAAAAJ
Sheryll Yohana Corchuelo Chavarro
author
position: Profesional Especializado
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: +57122077001262
email: scorchuelo@ins.gov.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
userId: http://scholar.google.com/citations?user=https://scholar.google.com/citations?hl=en&user=3LmURIwAAAAJ
Monica Marcela Benavides Ruiz
author
position: Líder de Laboratorio
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: +5712207700
email: marceb.ruiiz@gmail.com
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx
Marcela María Mercado Reyes
administrative point of contact
position: Subdirectora de Investigación Científica y Tecnológica
Instituto Nacional de Salud
Avenida calle 26 No. 51-20 - Zona 6 CAN
Bogotá
111321
Bogotá
CO
Telephone: 122077001315
email: mmercado@ins.gov.co
homepage: https://www.ins.gov.co/Paginas/Inicio.aspx