BASIDIOMYCETE YEASTS ISOLATED FROM PELTIGERA LICHENS OF SOUTHERN CHILE

Yosbany Perez; Nayla Serey; Ana María Millanes; Natalia Lizana; Katerin Almendras; Julieta Orlando; Yosbany Perez; Nayla Serey; Ana María Millanes; Natalia Lizana; Katerin Almendras; Julieta Orlando

doi:10.15468/hw7k9r

BASIDIOMYCETE YEASTS ISOLATED FROM PELTIGERA LICHENS OF SOUTHERN CHILE

Citation

Perez Y, Serey N, Millanes A M, Lizana N, Almendras K, Orlando J (2023). BASIDIOMYCETE YEASTS ISOLATED FROM PELTIGERA LICHENS OF SOUTHERN CHILE. Version 1.5. Instituto de Biodiversidad de Ecosistemas Antárticos y Subantárticos (BASE). Occurrence dataset https://doi.org/10.15468/hw7k9r accessed via GBIF.org on 2024-11-05.

Description

This resource represents to date the largest collection of basidiomycetes yeasts isolated from lichen thalli and the first collection of yeasts associated with Peltigera lichens. The Microbial Ecology Lab (Universidad de Chile) collection comprises 180 basidiomycetes yeasts isolated and identified from Peltigera lichen thalli (P. frigida, P. fuscopraetextata, P. rufescens and Peltigera ponojensis/monticola 11) collected from southern Chile. The isolates were identified by sequencing the ITS marker and subsequent phylogenetic analyses. A great diversity of basidiomycetes genera was found, belonging to the classes Agaricostilbomycetes, Cystobasidiomycetes, Microbotryomycetes and Tremellomycetes and to the orders Agaricostilbales, Cystobasidiales, Cystofilobasidiales, Filobasidiales, Holtermanniales, Sporidiobolales and Tremellales. Georeferencing included for all specimens.

Sampling Description

Study Extent

The samples of Peltigera species was collected in Region of Aysen del General Carlos Ibañez del Campo and Region of Magallanes y de la Antartica Chilena, Chile. The idendification of the yeast isoleted from the lichens was made betewen may and september of 2022

Sampling

Yeasts were isolated from thalli of four lichen species of the genus Peltigera (P. frigida, P. fuscopraetextata, Peltigera ponojensis/monticola 11, P. rufescens). The thalli were washed twice with sterile distilled water and subsequently macerated. They were seeded on three culture media (Yeast Malt Agar, Potato Dextrose Agar and a minimum agar medium without amino acids) supplemented with antibiotics. Cultures were incubated at 10°C in the dark for up to 60 days. Long-term preservation of yeast isolates was achieved at -20°C using cryotubes with sterile 15% glycerol and a dehydrated gelatin drop method at room temperature with sterile 10% gelatin (Marangon et al., 2003; Baeza et al., 2009). Taxonomic identification was performed by sequencing the ITS marker and subsequent phylogenetic analyses

Method steps

The thalli were washed twice with sterile distilled water and subsequently macerated.
They were seeded on three culture media (Yeast Malt Agar, Potato Dextrose Agar and a minimum agar medium without amino acids) supplemented with antibiotics.
Cultures were incubated at 10°C in the dark for up to 60 days.
Long-term preservation of yeast isolates was achieved at -20°C using cryotubes with sterile 15% glycerol and a dehydrated gelatin drop method at room temperature with sterile 10% gelatin.
Taxonomic identification was performed by sequencing the ITS marker and subsequent phylogenetic analyses.

Taxonomic Coverages

Fungi
rank: kingdom
Basidiomycota
rank: phylum
Agaricostilbomycetes
rank: class
Cystobasidiomycetes
rank: class
Microbotryomycetes
rank: class
Tremellomycetes
rank: class
Agaricostilbales
rank: order
Cystobasidiales
rank: order
Cystofilobasidiales
rank: order
Filobasidiales
rank: order
Holtermanniales
rank: order
Sporidiobolales
rank: order
Tremellales
rank: order
Bulleraceae
rank: family
Bulleribasidiaceae
rank: family
Chionosphaeraceae
rank: family
Cystobasidiaceae
rank: family
Filobasidiaceae
rank: family
Holtermanniaceae
rank: family
Mrakiaceae
rank: family
Piskurozymaceae
rank: family
Sporidiobolaceae
rank: family
Cystobasidium cf. laryngis
rank: species
Cystobasidium cf. pinicola
rank: species
Cystobasidium lysinophilum
rank: species
Cystobasidium ongulense
rank: species
Cystobasidium cf. psychroaquaticum
rank: species
Filobasidium stepposum
rank: species
Filobasidium wieringae
rank: species
Fonsecazyma cf. betulae
rank: species
Holtermanniella cf. wattica
rank: species
Naganishia adeliensis
rank: species
Naganishia onofrii
rank: species
Cystobasidium
rank: genus
Filobasidium
rank: genus
Fonsecazyma
rank: genus
Genolevuria
rank: genus
Goffeauzyma
rank: genus
Holtermanniella
rank: genus
Naganishia
rank: genus
Pseudotremella
rank: genus
Rhodotorula
rank: genus
Solicoccozyma
rank: genus
Tausonia
rank: genus
Rhodotorula cf. kratochvilovae
rank: species
Solicoccozyma gelidoterrea
rank: species
Solicoccozyma terricola
rank: species
Tausonia cf. pullulans
rank: species
Vishniacozyma cf. dimennae
rank: species
Vishniacozyma tephrensis
rank: species

Geographic Coverages

Region of Aysen del General Carlos Ibañez del Campo, Chile Region of Magallanes y de la Antártica Chilena, Chile

Bibliographic Citations

Baeza, M., Retamales, P., Sepúlveda, D., Lodato, P., Jiménez, A., & Cifuentes, V. (2009). Isolation, characterization and long term preservation of mutant strains of Xanthophyllomyces dendrorhous. J. Basic Microbiol., 49: 135-141. -
Marangon, A.V., Bertoni, T.A and Kioshima, E.S., (2003). Dehydrated gelatin drops: A good method for fungi maintenance and preservation. Microbiologica, 26, 305– 309. PMID: 12901428. -
Orlando J, Almendras K, Veas-Mattheos K, Pezoa M, Carú M (2023). Peltigera cyanolichens from Southern Chile. Version 1.5. Laboratory of Microbial Ecology (Laboratorio de Ecología Microbiana) - Universidad de Chile. Occurrence dataset https://doi.org/10.15468/ef76hj - https://doi.org/10.15468/ef76hj
Pérez, Y., Serey, N., Lizana, N., Millanes, A. M., Almendras, K., & Orlando, J. (2023). Primer reporte de levaduras basidiomicetes aisladas desde líquenes Peltigera del sur de Chile. XLIV Reunión anual de la Sociedad de Microbiología de Chile, La Serena, Coquimbo, Chile. Zenodo. https://doi.org/10.5281/zenodo.10424087 - https://doi.org/10.5281/zenodo.10424087
Pérez, Y., Serey, N., Millanes, A. M., Almendras, K., & Orlando, J. (2023). Diversidad de hongos aislados de talos de cianolíquenes Peltigera del sur de Chile. 3er Congreso Latinoamericano de Ecología Microbiana (ISME-Lat), Universidad Nacional de Quilmes, Bernal Buenos Aires, Argentina. Zenodo. https://doi.org/10.5281/zenodo.10420231 - https://doi.org/10.5281/zenodo.10420231
Pérez, Y.; Veas-Mattheos, K.; Almendras, K.; Orlando, J. Basidiomycete yeasts in lichens: a new component of lichen symbiosis?. ISME Latino Americano (ISME-LAT), Chile, 2021. -

Contacts

Yosbany Perez
originator
position: Postgraduate/PhD Candidate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56971331789
email: yosbany.perez@ug.uchile.cl

Nayla Serey
originator
position: Postgraduate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56962375844
email: nayla.serey@ug.uchile.cl

Ana María Millanes
originator
position: Full Professor
Departamento de Biología, Geología, Física y Química Inorgánica Universidad Rey Juan Carlos
C/ Tulipán, s/n E-28933
Móstoles
28933
Madrid
ES
Telephone: +34914888251
email: ana.millanes@urjc.es
homepage: http://biodiversos.org/en/staff/ana-maria-millanes-romero/

Natalia Lizana
originator
position: Undergraduate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56942738758
email: natalia.lizana@ug.uchile.cl

Katerin Almendras
originator
position: PhD
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56965378252
email: katalmendras@gmail.com

Julieta Orlando
originator
position: Associate Professor
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56229787401
email: jorlando@uchile.cl
homepage: https://www.institutobase.cl/

Yosbany Perez
metadata author
position: Postgraduate/PhD Candidate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56971331789
email: yosbany.perez@ug.uchile.cl

Nayla Serey
metadata author
position: Postgraduate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56962375844
email: nayla.serey@ug.uchile.cl

Ana María Millanes
metadata author
position: Full Professor
Departamento de Biología, Geología, Física y Química Inorgánica Universidad Rey Juan Carlos
C/ Tulipán, s/n E-28933
Móstoles
28933
Madrid
ES
Telephone: +34914888251
email: ana.millanes@urjc.es
homepage: http://biodiversos.org/en/staff/ana-maria-millanes-romero/

Natalia Lizana
metadata author
position: Undergraduate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56942738758
email: natalia.lizana@ug.uchile.cl

Katerin Almendras
metadata author
position: PhD
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56965378252
email: katalmendras@gmail.com

Julieta Orlando
metadata author
position: Associate Professor
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56229787401
email: jorlando@uchile.cl
homepage: https://www.institutobase.cl/

Yosbany Perez
originator
position: Postgraduate/PhD Candidate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56971331789
email: yosbany.perez@ug.uchile.cl

Nayla Serey
originator
position: Postgraduate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56962375844
email: nayla.serey@ug.uchile.cl

Ana María Millanes
originator
position: Full Professor
Departamento de Biología, Geología, Física y Química Inorgánica Universidad Rey Juan Carlos
C/ Tulipán, s/n E-28933
Móstoles
28933
Madrid
ES
Telephone: +34914888251
email: ana.millanes@urjc.es
homepage: http://biodiversos.org/en/staff/ana-maria-millanes-romero/

Natalia Lizana
originator
position: Undergraduate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56942738758
email: natalia.lizana@ug.uchile.cl

Katerin Almendras
originator
position: PhD
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56965378252
email: katalmendras@gmail.com

Julieta Orlando
originator
position: Associate Professor
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56229787401
email: jorlando@uchile.cl
homepage: https://www.institutobase.cl/

Juana Leal
administrative point of contact
position: Laboratory technician
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
email: reinajuanita@uchile.cl

Catalina Marín
reviewer
position: Data Manager
Instituto de Biodiversidad Antártica y Subantártica (BASE)
Victor Lamas 1290
Concepción
4030000
Bio Bio
CL
email: cmarin@institutobase.cl
homepage: https://www.institutobase.cl/

Julieta Orlando
administrative point of contact
position: Associate Professor
Univesidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56229787401
email: jorlando@uchile.cl
homepage: https://www.institutobase.cl/

Yosbany Perez
administrative point of contact
position: Postgraduate/PhD Candidate
Universidad de Chile
Las Palmeras 3425
Ñuñoa
7800003
Metropolitana
CL
Telephone: +56971331789
email: yosbany.perez@ug.uchile.cl