The study includes fungal genetic diversity assessment by ITS-1 next generation sequencing (NGS) analyses. The main the main experimental objects are soils of different classes, quality groups and also subjected to various agrotechnical treatments
Citation
MGnify (2020). The study includes fungal genetic diversity assessment by ITS-1 next generation sequencing (NGS) analyses. The main the main experimental objects are soils of different classes, quality groups and also subjected to various agrotechnical treatments. Sampling event dataset https://doi.org/10.15468/t4hl9h accessed via GBIF.org on 2024-11-09.Description
The study included fungal genetic diversity assessment by ITS-1 next generation sequencing (NGS) analyses as well as the characterization of the catabolic potential of microbial communities (Biolog EcoPlates) in the soil under long-term monoculture of maize using different cultivation techniques. The results obtained from the ITS-1 NGS technique enabled to classify and correlate the fungi species or genus to the soil metabolome. The research methods used in this paper have contributed to a better understanding of genetic diversity and composition of the population of fungi in the soil under the influence of the changes that have occurred in the soil under long-term maize cultivation. In all cultivation techniques, thThis study has shown that: (1) fungal diversity was changed under the influence different cultivation techniques; (2) techniques of maize cultivation and season were an important factors that can influence the biochemical activity of soil. Maize cultivated in direct sowing did not cause negative changes in the fungal structure, even making it more stable during seasonal changes; (3) full tillage and crop rotation may change fungal community and soil function. e season had a great influence on the fungal genetic structure in the soil.Sampling Description
Sampling
The study included fungal genetic diversity assessment by ITS-1 next generation sequencing (NGS) analyses as well as the characterization of the catabolic potential of microbial communities (Biolog EcoPlates) in the soil under long-term monoculture of maize using different cultivation techniques. The results obtained from the ITS-1 NGS technique enabled to classify and correlate the fungi species or genus to the soil metabolome. The research methods used in this paper have contributed to a better understanding of genetic diversity and composition of the population of fungi in the soil under the influence of the changes that have occurred in the soil under long-term maize cultivation. In all cultivation techniques, thThis study has shown that: (1) fungal diversity was changed under the influence different cultivation techniques; (2) techniques of maize cultivation and season were an important factors that can influence the biochemical activity of soil. Maize cultivated in direct sowing did not cause negative changes in the fungal structure, even making it more stable during seasonal changes; (3) full tillage and crop rotation may change fungal community and soil function. e season had a great influence on the fungal genetic structure in the soil.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/5.0
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
- Gałązka A, Grządziel J. 2018. Fungal Genetics and Functional Diversity of Microbial Communities in the Soil under Long-Term Monoculture of Maize Using Different Cultivation Techniques. Front Microbiol vol. 9 - DOI:10.3389/fmicb.2018.00076
Contacts
originatorInstitute of Soil Science and Plant Cultivation State Research Institute
metadata author
Institute of Soil Science and Plant Cultivation State Research Institute
administrative point of contact
Institute of Soil Science and Plant Cultivation State Research Institute