Nocardioides sambongensis Hwang, Son, Lee, He, Jo, Shin, and Ghim 2020
- Dataset
- Nocardioides sambongensis sp. nov., isolated from Dokdo Islands soil
- Rank
- SPECIES
- Published in
- Hwang, Ye-Ji, Son, Jin-Soo, Lee, Soo-Yeong, He, Yuxi, Jo, YoungJae, Shin, Jae-Ho, Ghim, Sa-Youl (2020): Nocardioides sambongensis sp. nov., isolated from Dokdo Islands soil. International Journal of Systematic and Evolutionary Microbiology 70 (1): 16-22, DOI: 10.1099/ijsem.0.003689, URL: http://dx.doi.org/10.1099/ijsem.0.003689
Classification
- kingdom
- Bacteria
- phylum
- Actinobacteria
- class
- Actinobacteridae
- order
- Actinomycetales
- family
- Nocardioidaceae
- genus
- Nocardioides
- species
- Nocardioides sambongensis
description
Cells are aerobic, Gram-stain-positive, non-motile and nonendospore-forming rods. The cells grow well under aerobic conditions on R 2 A agar and TSA plates. The individual cells are 0.5 – 0.6 × 0.8 – 1.0 µm in size. Colonies are circular, smooth, pale yellow in colour, and are approximately 1.0 – 2.5 mm in diameter after 2 days of incubation on R 2 A agar at 30 Ǫ C. Growth occurs between 25 and 37 Ǫ C, but not at 40 Ǫ C and above. The pH range for growth is pH 5 – 12 (optimally at pH 7 – 8). Grows in the presence of 0 – 7 % NaCl (w / v; optimally in 0.5 – 2 %), but not in 8 % NaCl. The cells are catalase- and oxidase-positive. The cells hydrolyse Tween 20, Tween 40, Tween 60 and Tween 80, but not casein, DNA, hypoxanthine, starch, L- tyrosine or xanthine. Cells are positive for nitrate reduction, aesculin hydrolysis, gelatin hydrolysisand glucose fermentation, but negative for indole production, arginine dihydrolase and urease (API 20 NE). D- Glucose is utilized, but adipate, L- arabinose, caproate, citrate, gluconate, N - acetyl-D- glucosamine, malate, maltose, D- mannitol, D- mannose and phenylacetate are not utilized as sole carbon and energy sources (API 20 NE). API 50 CH tests are positive for acid production from aesculin, cellobiose, glucose, 5 - ketogluconate, rhamnose, sucrose, trehalose, turanose and D- xylose, but negative for that from 2 - ketogluconate, adonitol, amygdalin, D- arabinose, D- arabitol, L- arabinose, L- arabitol, arbutin, dulcitol, erythritol, fructose, D- fucose, L- fucose, galactose, gluconate, glycerol, glycogen, inositol, inulin, lactose, maltose, mannitol, mannose, melezitose, melibiose, methyl a- D- glucoside, methyl a- D-mannoside, methyl b- D- xyloside, N - acetylglucosamine, gentiobiose, D- lyxose, D- tagatose, L- xylose raffinose, ribose, salicin, sorbitol, sorbose, starch and xylitol (API 50 CH). Test results are positive for cystine arylamidase, esterase, b-galactosidase, a- glucosidase, b- glucuronidase, naphthol-AS-BI-phosphohydrolase, trypsin and valine arylamidase, but negative for acid phosphatase, alkaline phosphatase, a-chymotrypsin, esterase lipase, a- fructosidase, a- galactosidase, b- glucosidase, leucine arylamidase, lipase, a- mannosidase and N - acetyl-b- glucosaminidase activities (API ZYM). The predominant menaquinone is MK- 8 (H 4). The major fatty acids are C 18: 1! 9 c, iso-C 16: 0 and iso-C. The DNA G + C content is 71.7 mol %.
etymology
Nocardioides sambongensis (sam. bong. en′ sis. N. L. masc. adj. sambongensis of or pertaining to Sambong Islands. Sambong, ancient name of the Dokdo Islands, located on the coast of the East Sea in Korea, from where the organism was isolated).
materials_examined
The type strain is KUDC 5002 T (= KCTC 39855 T = DSM 106604 T), which was isolated from soil collected from the Dokdo Islands, Republic of Korea. The 16 S rRNA gene sequence and the whole genome sequence of strain KUDC 5002 T has been deposited in GenBank / EMBL / DDBJ under the accession numbers KX 858534 and CP 041091. General features of the genome assembly are as follows: genome size, 4 434 294 bp; number of contigs, 1; coverage, 111.0 ×.