Aeolian microbial dispersal limitation to Antarctica (2017)
Citation
Archer S, Maestre F, Maki T, Lee C, Cowan D, Pointing S, Sweetlove M (2021). Aeolian microbial dispersal limitation to Antarctica (2017). SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/ktwewp accessed via GBIF.org on 2024-09-01.Description
Amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria (16S ssu rRNA) and Fungi (ITS) in samples (n=110) from soils and aerosol in the McMurdo Dry Valleys and New Zealand.Sampling Description
Study Extent
Air mass at near-surface (1.5m above ground) and underlying soil was sampled from 11 to 23 January 2017 at valley and elevated locations throughout the Wright Valley, McMurdo Dry Valleys, Antarctica. Comparison samples were taken from the North Island of New Zealand.Sampling
Sampling of air mass above the boundary layer for surface influence was achieved by mounting the apparatus in a helicopter with an external sampling port.Method steps
- Total DNA was directly extracted using a CTAB protocol. DNA yield for these ultra-low biomass samples was quantified using the Qubit 2.0 Fluorometer (Invitrogen) in the range 1.06-8.44ng. Samples were then stored at -20 C. Illumina MiSeq libraries were prepared as per manufacturer’s protocol (Metagenomic Sequencing Library Preparation Part # 15044223 Rev. B; Illumina, San Diego, CA, USA) and with PhiX positive controls. Bacteria and Fungi were targeted with domain-specific PCR primers PCR1 forward (5’ TCGTCGGCAGCGTCAGATGT GTATAAGAGA CAGCCTACGG GNGGCWGCAG 3’) and PCR1 reverse (5’ GTCTCGTGGG CTCGGAGATG TGTATAAGAG ACAGGACTAC HVGGGTATCT AATCC 3’) and the internal transcribed spacer region of fungal 18S and 5.8S rRNA genes: ITS1 forward (5' CTTGGTCATTTAGAGGAAGTAA 3') and ITS2 reverse (5' GCTGCGTTCTTCATCGATGC 3').
Taxonomic Coverages
Geographic Coverages
Antarctica:McMurdo Dry Valleys:Bull Pass, Valley ridge; New Zealand:Glen Eden, Auckland
Bibliographic Citations
- Archer, S., Lee, K., Caruso, T., Maki, T., Lee, C., Cowan, D., ... & Pointing, S. (2018). Microbial dispersal limitation to isolated soil habitats in the McMurdo Dry Valleys of Antarctica. bioRxiv, 493411. - https://doi.org/10.1101/493411
Contacts
Stephen Archeroriginator
Institute for Applied Ecology New Zealand
Auckland
NZ
Fernando Maestre
originator
Universidad Rey Juan Carlos
Mostoles
ES
Teruya Maki
originator
Kanazawa University
Kanazawa
JP
Charles Lee
originator
University of Waikato
Hamilton
NZ
Don Cowan
originator
University of Pretoria
Pretoria
ZA
Stephen Pointing
originator
Institute for Applied Ecology New Zealand
Auckland
NZ
Maxime Sweetlove
metadata author
Royal Belgian Institute of Natural Sciences
Brussels
BE
email: msweetlove@naturalsciences.be
Stephen Archer
user
Institute for Applied Ecology New Zealand
Auckland
NZ
Stephen Archer
administrative point of contact
Institute for Applied Ecology New Zealand
Auckland
NZ