Nocardioides vastitatis Liu, Xue, Li, and Sun 2020
- Dataset
- Nocardioides vastitatis sp. nov., isolated from Taklamakan desert soil
- Rank
- SPECIES
- Published in
- Liu, Shao-Wei, Xue, Chun-Mei, Li, Fei-Na, Sun, Cheng-Hang (2020): Nocardioides vastitatis sp. nov., isolated from Taklamakan desert soil. International Journal of Systematic and Evolutionary Microbiology 70 (1): 77-82, DOI: 10.1099/ijsem.0.003718, URL: http://dx.doi.org/10.1099/ijsem.0.003718
Classification
- kingdom
- Bacteria
- phylum
- Actinobacteria
- class
- Actinobacteridae
- order
- Actinomycetales
- family
- Nocardioidaceae
- genus
- Nocardioides
- species
- Nocardioides vastitatis
description
Cells are Gram-stain-positive, aerobic, non-motile, nonspore-forming and rod-shaped (approximately 0.4 – 0.7 × 1.1 – 2.3 µm). Colonies on ISP 2 agar are circular, smooth, opaque, creamy white in colour. Grows well on ISP 2, TSA, R 2 A agar and nutrient agar. Poor growth occurs on ISP 3, ISP 4, ISP 5 and ISP 7 agar. Growth occurs at 10 – 37 Ǫ C (optimum, 28 – 30 Ǫ C), pH 6.0 -- 9.0 (optimum, pH 7.0) and with NaCl concentrations of 0 – 3 % (w / v; optimum, 0 %). Catalase-positive and oxidase-negative. Hydrolyses Tween 20 and Tween 40, but does not hydrolyse casein, gelatin, Tween 80, carboxymethyl-cellulose, skimmed milk, starch and aesculin. Negative for nitrate reduction, indole production, glucose fermentation, H 2 S production, urease and arginine dihydrolase. Assimilates D-mannitol and malate. Does not assimilate D- glucose, L- arabinose, D- mannose, N - acetyl-D- glucosamine, maltose, potassium gluconate, caprate, adipate, citrate and phenylacetate. Positive for alkaline phosphatase, esterase (C 4), esterase lipase (C 8), leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, b- galactosidase, a- glucosidase and b- glucosidase, but negative for lipase (C 14), valine arylamidase, cysteine arylamidase, trypsin, a- chymotrypsin, a- galactosidase, b- glucuronidase, N - acetyl-b- glucosaminidase, a- mannosidase and a- fucosidase. Acids are produced from L- arabinose, D- xylose (weakly), D- glucose, D- fructose, L- rhamnose, inositol, mannitol, aesculin, sucrose (weakly), inulin (weakly) and raffinose (weakly). The cell-wall peptidoglycan contains LL- diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone is MK- 8 (H 4). The polar lipids comprise DPG, PG, PI, an unidentified phospholipid and three unidentified lipids. The major whole-cell fatty acids (> 10 %) are iso-C 16: 0 and 10 - methyl C 18: 0 (TBSA). The G + C content of the genomic DNA is 70.4 mol %.
etymology
Nocardioides vastitatis (vas. ti. ta′ tis. L. masc. gen. n. vastitatis of a desert).
materials_examined
The type strain, 21 Sc 5 - 5 T (= JCM 33365 T = CGMCC 4.76 08 T), was isolated from soil sampled at the rhizosphere of Inula salsoloides collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China.