Maximising fish detection with eDNA metabarcoding, Browse Island, Australia (2017)
Citation
Bessey, Cindy, Simon N. Jarman, Oliver Berry, Ylva S. Olsen, Michael Bunce, Tiffany Simpson, Matthew Power, James McLaughlin, Graham J. Edgar, and John Keesing. "Maximizing Fish Detection with eDNA Metabarcoding." Environmental DNA (February 20, 2020). doi:10.1002/edn3.74. accessed via GBIF.org on 2024-08-17.Description
Water samples were collected from the intertidal reef of Browse Island; a tropical, remote island in the Timor Sea. Aliquots from a single 20700 ml sample and multiple 2000 ml samples were filtered in various volumes (25 ml to 2000 ml) across two membrane sizes (0.20 µm and 0.45 µm). A fish metabarcoding assay was used to characterise the fish diversity within aliquots. All samples, except one, yielded fish DNA sequences. Two hundred and nine operational taxonomic units (cf. species) representing 48 fish families were identified from the complete collection of DNA contained in all samples, comparable to the 200 fish species detected using conventional surveys at this location.
Methods are decsribed in the cited paper Bessey et al. "Maximizing Fish Detection with eDNA Metabarcoding." Environmental DNA (February 20, 2020). doi:10.1002/edn3.74
Data accessed from the Atlas of Living Australia (ALA) 'Maximising fish detection with eDNA metabarcoding' collection at https://collections.ala.org.au/public/show/dr14581 on 2021-12-01
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
Contacts
Cindy Bessyoriginator
position: Principal Investigator
CSIRO Oceans and Atmosphere
64 Fairway
Crawley
6009
Western Australia
AU
email: Cindy.Bessey@csiro.au
Cindy Bessy
metadata author
position: Principal Investigator
CSIRO Oceans and Atmosphere
64 Fairway
Crawley
6009
Western Australia
AU
email: Cindy.Bessey@csiro.au
OBIS Australia Node manager
publisher
position: OBIS Australia Data Manager
CSIRO National Collections and Marine Infrastructure Data Centre
Castray Esplande
Hobart
7000
Tasmania
AU
email: obisau@csiro.au
homepage: http://www.obis.org.au
Cindy Bessy
administrative point of contact
position: Principal Investigator
CSIRO Oceans and Atmosphere
64 Fairway
Crawley
6009
Western Australia
AU
email: Cindy.Bessey@csiro.au