Environmental Foraminifera Raw sequence reads
Citation
MGnify (2019). Environmental Foraminifera Raw sequence reads. Sampling event dataset https://doi.org/10.15468/zhhrft accessed via GBIF.org on 2024-08-26.Description
Paleotsunami deposits are primary source of information on past big tsunami events and thereby are critical for earthquake and tsunami hazard assessment. They usually form sandy layers preserved in coastal sediments that may contain indicators of marine origin such as foraminifera microfossils and the geochemical signal of saltwater. Environmental DNA sequencing of marine species targets in a series of up to about 2000 years old sandy paleotsunami deposits is promising to detect ancient tsunami events even in the absence of microfossil and sedimentological evidence.The DNA content of 10 environmental coastal sediment samples was extacted from paleotsunami deposit and peat layers of a core and additional peat and beach sand surface samples taken from a wetland on eastern Hokkaido Island (Japan) facing the Kuril Trench subduction zone generating frequent large tsunamigenic earthquakes. The 37f hypervariable region of the foraminiferal SSU rDNA was PCR amplified and PCR products were pooled in one library for Illumiona MiSeq sequence and diversity analyses.Sampling Description
Sampling
Paleotsunami deposits are primary source of information on past big tsunami events and thereby are critical for earthquake and tsunami hazard assessment. They usually form sandy layers preserved in coastal sediments that may contain indicators of marine origin such as foraminifera microfossils and the geochemical signal of saltwater. Environmental DNA sequencing of marine species targets in a series of up to about 2000 years old sandy paleotsunami deposits is promising to detect ancient tsunami events even in the absence of microfossil and sedimentological evidence.The DNA content of 10 environmental coastal sediment samples was extacted from paleotsunami deposit and peat layers of a core and additional peat and beach sand surface samples taken from a wetland on eastern Hokkaido Island (Japan) facing the Kuril Trench subduction zone generating frequent large tsunamigenic earthquakes. The 37f hypervariable region of the foraminiferal SSU rDNA was PCR amplified and PCR products were pooled in one library for Illumiona MiSeq sequence and diversity analyses.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
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Bibliographic Citations
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originatorUniversity of Geneva
metadata author
University of Geneva
administrative point of contact
University of Geneva