EDP Foz Tua – Aquatic taxa recorded during fish sampling for population genetic analysis (Complementary Studies – Element 24) [2017]

Study Extent

The data were collected along the River Tua (Northeast Portugal) and two of its main tributaries (Tinhela and Tinhela), up to 52 kms from the mouth, in the municipalities of Alijó, Carrazeda de Ansiães, Murça, Vila Flor, Mirandela, Macedo de Cavaleiros and Vila Pouca de Aguiar, and in rivers Torto, Távora, Tedo and Pinhão, in the Douro basin, in the municipalities of S. João da Pesqueira, Tabuaço and Armamar.

Sampling

The harvesting at each location were carried out using electric fishing, based on standardized procedures (INAG, 2008). The fishes’ captures were carried out against the current, in the downstream-upstream direction, involving a team of four elements, including an operator of the electric fishing net, an assistant with a hand-held net to collect individuals, and two assistants to ensure safety and help in sample processing. In each location, a river section of about 50-100m in length was prospected, and individuals of the target species captured were kept. Priority was given to adult individuals of each species, collected as much as possible from different points of the river, in order to reduce the risk of collection of related individuals who could introduce bias in the genetic analyses. From each individual, a small portion of tissue from the dorsal fin was cut, stored in an eppendorf tube with ethanol analysis (96%) and properly labeled. Each individual was also measured (total length) and photographed. Collection methods and conservation have already been properly tested in previous works by CIBIO-InBIO. At the end of the campaign, the samples collected were kept in stable temperature conditions, stored and transported to the laboratory. The locations of all samples were georeferenced with the aid of a GPS device and subsequently processed in a GIS environment.

Quality Control

All identifications were performed by qualified technicians

Method steps

1. To be completed

The taxonomic coverage of this dataset spans 2 phyluns, 3 classes, 5 orders, 2 families, 5 genera and 6 species.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Chordata
   rank: phylum
4. Insecta
   rank: class
5. Actinopterygii
   rank: class
6. Mammalia
   rank: class
7. Ephemeroptera
   rank: order
8. Plecoptera
   rank: order
9. Trichoptera
   rank: order
10. Cypriniformes
    rank: order
11. Soricomorpha
    rank: order
12. Cyprinidae
    rank: family
13. Talpidae
    rank: family

The data were collected along the River Tua (Northeast Portugal) and two of its main tributaries (Tinhela and Tinhela), up to 52 kms from the mouth, in the municipalities of Alijó, Carrazeda de Ansiães, Murça, Vila Flor, Mirandela, Macedo de Cavaleiros and Vila Pouca de Aguiar, and in rivers Torto, Távora, Tedo and Pinhão, in the Douro basin, in the municipalities of S. João da Pesqueira, Tabuaço and Armamar.