anchialine metagenome Raw sequence reads
Citation
MGnify (2020). anchialine metagenome Raw sequence reads. Sampling event dataset https://doi.org/10.15468/4s5r6q accessed via GBIF.org on 2024-08-24.Description
Microbial ecology is an important and growing field that has revealed the importance of microbes in many ecosystems; however, some ecosystems have yet to receive much attention including the anchialine ecosystem (nearshore bodies of water with subsurface freshwater and seawater connections). Hawaiian anchialine habitats in the Cape Kinau region of Maui and the Kona region of Hawaii exhibit distinctive, laminated orange cyanobacterial-bacterial crusts, but little is known about their composition, the composition of microbial communities in non-crust containing sites lacking the cyanobacterial-bacterial crusts, or the degree to which environmental factors structure them. During the summer of 2010, we surveyed benthic and water column microbial communities from ten anchialine habitats on Oahu, Maui, and Hawaii using high-throughput amplicon sequencing of the V6 (prokaryotic-biased) and V9 (eukaryotic-biased) hypervariable regions of the SSU rRNA gene. A subset of six anchialine habitats were also sampled in the spring, summer, and winter of 2011 to allow elucidation of seasonal effects on the Hawaiian anchialine microbial community.Sampling Description
Sampling
Microbial ecology is an important and growing field that has revealed the importance of microbes in many ecosystems; however, some ecosystems have yet to receive much attention including the anchialine ecosystem (nearshore bodies of water with subsurface freshwater and seawater connections). Hawaiian anchialine habitats in the Cape Kinau region of Maui and the Kona region of Hawaii exhibit distinctive, laminated orange cyanobacterial-bacterial crusts, but little is known about their composition, the composition of microbial communities in non-crust containing sites lacking the cyanobacterial-bacterial crusts, or the degree to which environmental factors structure them. During the summer of 2010, we surveyed benthic and water column microbial communities from ten anchialine habitats on Oahu, Maui, and Hawaii using high-throughput amplicon sequencing of the V6 (prokaryotic-biased) and V9 (eukaryotic-biased) hypervariable regions of the SSU rRNA gene. A subset of six anchialine habitats were also sampled in the spring, summer, and winter of 2011 to allow elucidation of seasonal effects on the Hawaiian anchialine microbial community.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
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Contacts
originatorAuburn University
metadata author
Auburn University
administrative point of contact
Auburn University