The InBIO Barcoding Initiative Database: Hemiptera 01

Study Extent

Specimens were collected in 60 different localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions.

Sampling

The studied material was collected in 60 different localities from continental Portugal. Sampling was conducted between 2015 and 2019 on a wide range of habitats, both by direct search of specimens or using a hand-held sweep net. Collected specimens were examined using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature.

Quality Control

All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org).

Method steps

1. Specimens were collected in 60 different localities of continental Portugal. Fieldwork was carried out between 2015 and 2019.
2. Specimens were collected during fieldwork by direct search of specimens or by sweeping the vegetation and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
3. Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature. A subset (23%) was identified using the BOLD Identification Engine directly.
4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For one specimens of Ceraleptus lividus only a 418 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
5. All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

This dataset is composed of data relating to 135 Hemiptera specimens. All specimens were determined to species level or below. Overall, 90 species and one subspecies are represented in the dataset. These species belong to 31 families. The Pentatomidae family accounts for 20% of the total collected specimens, and no other family accounts for more than 8%. The Pentatomidae and Miridae families combined account for 26% of the total taxa represented, and no other family accounts for more than 7%. Eleven families are represented by a single taxon and ten by two taxa.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Hemiptera
   rank: order
5. Acanthosomatidae
   rank: family
6. Alydidae
   rank: family
7. Aphalaridae
   rank: family
8. Aphrophoridae
   rank: family
9. Aradidae
   rank: family
10. Auchenorrhyncha
    rank: family
11. Berytidae
    rank: family
12. Cercopidae
    rank: family
13. Cicadellidae
    rank: family
14. Cicadidae
    rank: family
15. Coreidae
    rank: family
16. Corixidae
    rank: family
17. Cydnidae
    rank: family
18. Delphacidae
    rank: family
19. Dictyopharidae
    rank: family
20. Flatidae
    rank: family
21. Gerridae
    rank: family
22. Heteroptera
    rank: family
23. Hydrometridae
    rank: family
24. Lygaeidae
    rank: family
25. Membracidae
    rank: family
26. Miridae
    rank: family
27. Nabidae
    rank: family
28. Nepidae
    rank: family
29. Notonectidae
    rank: family
30. Pentatomidae
    rank: family
31. Potamocoridae
    rank: family
32. Pyrrhocoridae
    rank: family
33. Reduviidae
    rank: family
34. Rhopalidae
    rank: family
35. Rhyparochromidae
    rank: family
36. Stenocephalidae
    rank: family
37. Thaumastocoridae
    rank: family

Continental Portugal.