Pareuzebyella sediminis Huang, Wei, Lai, Chen, and Yuan 2021
- Dataset
- PareUZebyella sediminis gen. nov., sp. nov., a novel marine bacterium in the family FlaVObaCTeriaCeae, isolated from a tidal flat sediment
- Rank
- SPECIES
- Published in
- Huang, Zhaobin, Wei, Xiaomei, Lai, Qiliang, Chen, Shiyong, Yuan, Jianjun (2021): PareUZebyella sediminis gen. nov., sp. nov., a novel marine bacterium in the family FlaVObaCTeriaCeae, isolated from a tidal flat sediment. International Journal of Systematic and Evolutionary Microbiology (004606) 71 (1): 1-9, DOI: 10.1099/ijsem.0.004606, URL: http://dx.doi.org/10.1099/ijsem.0.004606
Classification
- kingdom
- Bacteria
- phylum
- Bacteroidetes
- class
- Flavobacteriia
- order
- Flavobacteriales
- family
- Flavobacteriaceae
- genus
- Pareuzebyella
- species
- Pareuzebyella sediminis
description
Cells are approximately 0.5 µm in width and 1.6 µm in length. Colonies on MB agar plate are light-orange, round, and small. Growth occurs at the temperature of 15 – 40 ° C with optimum of 30 – 35 ° C. No growth was found at 10 or 45 ° C. NaCl requirement range was found to be 0.5 – 10 %, with optimum of 1 – 3 %. The species can degrade Tween 40 and Tween 60, but showed no ability to degrade skim milk (protein), cellulose, starch, and Tween 80. Nitrate cannot be reduced into nitrite. Positive for alkaline phosphatase, esterase (C 4), esterase lipase (C 8), lipase (C 14), leucine arylamidase, valine arylamidase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-Phosphohydrolase, β - galactosidase and α - glucosidase; weak positive for α - mannosidase and tryptophan deaminase, and negative for trypsin, α - chymotrypsin, α - galactosidase, β - glucuronidase, β - glucosidase, N-acetylβ - glucosaminidase, and β - fucosidase. Gelatin and aesculin can be hydrolysed. Voges-Proskauer reaction is positive. The predominant menaquinone is MK- 6. The predominant fatty acids are C 15: 0 iso, C 17: 0 iso 3 - OH, C 15: 1 iso G, and summed feature 9 (C 17: 1 iso ω 9 c and / or C 16: 0 10 - methyl). The polar lipid profiles included phosphatidylethanolamine (PE), four unidentified aminolipids (AL) and four unidentified lipids. The genome size is 4.9 – 5.0 Mb with genomic G + C content of 41.5 mol %. The type strain, S 2 - 4 - 21 T (= MCCC 1 K 03818 T = KCTC 72152 T) was isolated from the sediment of coastal cordgrass S. alterniflora and another strain MT 2 - 5 - 19 (= KCTC 72539 = MCCC 1 K 03874) was isolated from the neighbouring oyster field in Quanzhou bay, China.
etymology
Pareuzebyella sediminis (se. di'mi. nis. L. gen. n. sediminis of sediment).
materials_examined
The GenBank / EMBL / DDBJ accession numbers for the 16 S rRNA gene sequences of strain S 2 - 4 - 21 T and MT 2 - 5 - 19 are MN 227660 and MN 227661, respectively. The whole genome shotgun projects of strain S 2 - 4 - 21 T and MT 2 - 5 - 19 has been deposited at DDBJ / ENA / GenBank under the accessions of VTZS 00000000 and VTZT 00000000, respectively.