Dyella dinghuensis Ou, Gao, Chen, Bi, and Qiu 2019
- Dataset
- Dyella dinghuensis sp. nov. and Dyella choica sp. nov., isolated from forest soil
- Rank
- SPECIES
- Published in
- Ou, Fang-hong, Gao, Zeng-hong, Chen, Mei-hong, Bi, Jie-yi, Qiu, Li-hong (2019): Dyella dinghuensis sp. nov. and Dyella choica sp. nov., isolated from forest soil. International Journal of Systematic and Evolutionary Microbiology 69 (5): 1496-1503, DOI: 10.1099/ijsem.0.003356, URL: http://dx.doi.org/10.1099/ijsem.0.003356
Classification
- kingdom
- Bacteria
- phylum
- Proteobacteria
- class
- Gammaproteobacteria
- order
- Xanthomonadales
- family
- Xanthomonadaceae
- genus
- Dyella
- species
- Dyella dinghuensis
description
Cells are Gram-stain-negative, aerobic, non-motile, and rod-shaped (0.3 – 0.6 × 1.0 – 2.3 µm). Colonies are circular, convex with clear margin and yellow-coloured after 4 days on R 2 A agar. Growth was observed on trypticase soy agar, R 2 A agar and Luria – Bertani agar, but not MacConkey agar. Growth on R 2 A agar occurs at 12 – 37 Ǫ C, pH 4.5 – 7.0 and in the presence of 0 – 1.0 % (w / v) NaCl. Optimum growth occurs at 28 Ǫ C, pH 5.0 – 6.0 and without NaCl supplement. Catalase-positive and oxidase-positive. Nitrate is not reduced to nitrite. Casein is hydrolysed, but gelatin, starch and Tween 80 are not. H 2 S and indole are not produced. The following substrates can be used as sole carbon sources for growth: adipic acid, arbutin, amygdalin, D- xylose, D- galactose, D- glucose, D- fructose, D- mannose, D- mannitol, cellobiose, maltose, inulin, malic acid, methyl Oi- D- glucopyranoside, N - acetyl-D- glucosamine and phenylacetic acid. The following substrates are not utilized for growth: aesculin ferric citrate, capric acid, D- adonitol, D- arabinose, D- arabitol, D- fucose, D- gentiobiose, lactose, D- lyxose, melezitose, melibiose, raffinose, D- ribose, D- sorbitol, sucrose, D- tagatose, trehalose, turanose, dulcitol, erythritol, glycerol, glycogen, inositol, L- arabinose, L- arabitol, L- fucose, L- rhamnose, L- sorbose, L- xylose, methyl Oi - D- mannopyranoside, methyl β - D- xylopyranoside, potassium 2 - ketogluconate, potassium 5 - ketogluconate, potassium gluconate, salicin, starch and xylitol. The following enzyme activities are positive: alkaline phosphatase, acidphosphatase, arginine dihydrolase, cystine arylamidase, Oi - galactosidase, Oi - glucosidase, Oi - fucosidase, Oi - mannosidase, β - galactosidase, β - glucosidase, esterase (C 4), esterase (C 8), leucine arylamidase, naphthol-AS-BIphosphohydrolase, N - acetyl- β - glucosaminidase, urease and valine arylamidase. Trypsin is weakly positive. The following enzyme activities are negative: Oi - chymotrypsin, β - glucuronidase and lipase (C 14). Ubiquinone- 8 is the only respiratory quinone, and iso-C 15: 0, iso-C 16: 0, iso-C 17: 0, iso- C 17: 1 Ɯ 9 c are the major cellular fatty acids (> 10 %). The G + C content of the genomic DNA is 59.1 mol %. The major polar lipids consist of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, one unidentified aminophospholipid, two unidentified aminolipids, one unidentified phospholipid and lipid. The type strain, DHOA 06 T (= KCTC 52129 T = NBRC 111978 T), was isolated from forest soil sampled at Dinghushan Biosphere Reserve, Guangdong Province, PR China. The 16 S rRNA gene sequence and whole genome sequence of strain DHOA 06 T have been deposited in DDBJ / EMBL / GenBank under accession numbers KY 194795 and RYZR 00000000, respectively.
etymology
Dyella dinghuensis (ding. hu. en′ sis. N. L. fem. adj. dinghuensis referring to Dinghu Mountain, where the organism was isolated).