Fundicoccus IGNAVUS 2020
- Dataset
- Fundicoccus ignavus gen. nov., sp. nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk
- Rank
- GENUS
- Published in
- Siebert, Annemarie, Huptas, Christopher, Wenning, Mareike, Scherer, Siegfried, Doll, Etienne V. (2020): Fundicoccus ignavus gen. nov., sp. nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk. International Journal of Systematic and Evolutionary Microbiology 70 (8): 4774-4781, DOI: 10.1099/ijsem.0.004344, URL: http://dx.doi.org/10.1099/ijsem.0.004344
Classification
- kingdom
- Bacteria
- phylum
- Firmicutes
- class
- Bacilli
- order
- Lactobacillales
- family
- Aerococcaceae
- genus
- Fundicoccus
description
Cells are circular-shaped (cocci) with an approximate size of 1 – 2 µm. They occur as single cells and form pairs or chains. Cells are Gram-stain-positive, non-motile, non-endospore-forming, oxidase- and catalase-negative, and facultatively anaerobic. The isolates show strong α- haemolysis on 5 % sheep blood agar. The optimal growth conditions are at 29 – 33 ° C, pH 7.5 – 8.0 and 0.5 – 1 % (w / v) NaCl. Growth occurs at 12 – 39 ° C with one strain only growing up to 37 ° C (WS 5303). Strains grow at pH 6.5 – 9.5 and strain WS 4937 T as well as WS 4753 are able to grow in the presence of up to 6.5 % (w / v) NaCl. Acid formation was not detected for any of the 47 carbohydrates tested with the API 50 CH system. Does not hydrolyse starch, gelatine or aesculin. Negative for the Voges – Proskauer test and indole production. Nitrate is not reduced. H 2 S is not detected. Positive for argininedihdyrolase, β - galactosidase activity (only with resorufin β - Dgalactopyranoside as substrate) and hydrolysis of hippurate. Alanyl-phenylalanyl-proline-arylamidase, alkaline phosphatase, β - glucuronidase, β - mannosidase, glycyl-tryptophanarylamidase, N - acetyl- β - glucosaminidase, pyroglutamic acid arylamidase and urease are not produced. α - Glucosidase is weakly positive for WS 4937 T. The phenotype for amino acid catabolism was supported by genome data. Additionally, an orthologue of the alkaline phosphatase was detected which is not active under conditions used in phenotypic experiments. The production of β - glucosidase is variable but was detected for the type strain WS 4937 T and strain WS 5303. The major fatty acids are C 16: 1 ω 9 с, C 16: 0 and C 18: 1 ω 9 с. The polar lipid profile consists of three glycolipids, phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol.
etymology
Fundicoccus ignavus (ig. na’vus. L. masc. adj. ignavus inactive, referring to the lack of acid formation from carbohydrates).
materials_examined
The type strain of Fundicoccus ignavus gen. nov., sp. nov. is WS 4937 T (= DSM 109652 T = LMG 31441 T), which was isolated from raw milk of a farm bulk tank. The DNA G + C content of the type strain is 37.4 mol % and the genome size is ~ 3.0 Mb. WS 5303 (= DSM 109653 = LMG 31442) and WS 4759, two additional strains of the species were isolated from other bulk tank milk samples. Whole-genome sequences of WS 4937 T, WS 4759 and WS 5303 can be accessed with WJQT 00000000, WJQS 00000000 and WJQR 00000000, respectively.